Separation of catechins and methylxanthines in tea samples by capillary electrochromatography
| Autor: | Zeineb Aturki, Maria Augusta Raggi, Salvatore Fanali, Ülkü Dilek Uysal |
|---|---|
| Přispěvatelé: | Anadolu Üniversitesi, Fen Fakültesi, Kimya Bölümü, Uysal, Ülkü Dilek, U. Dilek Uysal, Z. Aturki, M.A. Raggi, S. Fanali |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Analyte
Capillary electrochromatography Chromatography Resolution (mass spectrometry) Molecular Structure Tea Extraction (chemistry) Filtration and Separation Hydrogen-Ion Concentration Catechin Analytical Chemistry Catechins chemistry.chemical_compound chemistry Electrochromatography Capillary Electrochromatography Phase (matter) Xanthines Linear Models Methylxanthines Quantitative analysis (chemistry) Ammonium acetate Tea Samples Rp-Cec |
| Zdroj: | 33rd International Symposium on Capillary Chromatography & Electrophoresis, portland, Oregon, USA, 2009 info:cnr-pdr/source/autori:U. D. Uysal, Z. Aturki, M. A. Raggi, S. Fanali/congresso_nome: 33rd International Symposium on Capillary Chromatography & Electrophoresis/congresso_luogo:portland, Oregon, USA/congresso_data:2009/anno:2009/pagina_da:/pagina_a:/intervallo_pagine Journal of separation science 32 (2009): 1002–1010. doi:10.1002/jssc.200800634 info:cnr-pdr/source/autori:Ulku Dilek Uysal (1); Zeineb Aturki (1); Maria Augusta Raggi (2); Salvatore Fanali (1)/titolo:Separation of catechins and methylxanthines in tea samples by capillary electrochromatography./doi:10.1002%2Fjssc.200800634/rivista:Journal of separation science (Print)/anno:2009/pagina_da:1002/pagina_a:1010/intervallo_pagine:1002–1010/volume:32 |
| DOI: | 10.1002/jssc.200800634 |
| Popis: | PubMed: 19266552 In this paper, the simultaneous separation of several polyphenols such as (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, theophylline, caffeine in green and black teas by capillary electrochromatography (CEC) was developed. Several experimental parameters such as stationary phase type, mobile phase composition, buffer and pH, inner diameter of the columns, sample injection, were evaluated to obtain the complete separation of the analysed compounds. Baseline resolution of the studied polyphenols was achieved within 30 min by using a capillary column (id 100 µm) packed with bidentate C18 particles for 24.5 cm and a mobile phase composed of 5 mM ammonium acetate buffer pH 4 with H2O/ACN (80:20, v/v). The applied voltage and the temperature were set at 30 kV and 20°C. Precision, detection and quantification limits, linearity, and accuracy were investigated. A good linearity (R2 > 0.9992) was achieved over a concentration working range of 2-100 µg/mL for all the analytes. LOD and LOQ were 1 and 2 µg/mL, respectively, for all studied compounds. The CEC method was applied to the analysis of those polyphenols in green and black tea samples after an extraction procedure. Good recovery data from accuracy studies ranged between 90% and 112% for all analytes |
| Databáze: | OpenAIRE |
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