Factors that influence phosphoenolpyruvate-induced calcium efflux from rat liver mitochondria
| Autor: | C. Bhuvaneswaran, D.W. Price, C.F. Peng, C.L. Wadkins |
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| Rok vydání: | 1974 |
| Předmět: |
Male
Organomercury Compounds DTNB education Carboxylic Acids Biophysics chemistry.chemical_element Mitochondria Liver In Vitro Techniques Biology Calcium Benzoates Biochemistry Phosphates Phosphoenolpyruvate chemistry.chemical_compound Adenosine Triphosphate Oxygen Consumption Adenine nucleotide Pseudomonas Animals Disulfides Molecular Biology Toxins Biological Adenine Nucleotides Calcium Radioisotopes Biological Transport Cell Biology Nitro Compounds Mersalyl Rats chemistry Ethylmaleimide Mitochondrial matrix cardiovascular system Efflux Phosphoenolpyruvate carboxykinase Adenosine triphosphate Polarography circulatory and respiratory physiology |
| Zdroj: | Biochemical and Biophysical Research Communications. 56:134-141 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(74)80325-6 |
| Popis: | Summary Stimulation by phosphoenolpyruvate (PEP) of Ca 2+ efflux from preloaded rat liver mitochondria is associated with an enhanced respiratory rate and is dependent on the P i concentration of the extramitochondrial medium. PEP-induced Ca 2+ efflux is inhibited by concentrations of NEM, mersalyl, and DTNB that also inhibit the respiration-linked transport of P i as well as by bongkrekic acid at concentrations that also inhibits the adenine nucleotide translocase. Since PEP can gain access to the mitochondrial matrix by means of a carrier mediated exchange with intramitochondrial adenine nucleotides, we conclude that the ability of PEP to induce Ca 2+ efflux could be associated with adenine nucleotide efflux as well as P i influx and that the stability of the accumulated Ca 2+ is likely to be controlled by the intramitochondrial ATP/P i ratio. |
| Databáze: | OpenAIRE |
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