The ratio of physical particles per infectious unit observed for poliomyelitis viruses

Autor:	Jørgen Fogh, Carlton E. Schwerdt
Rok vydání:	1957
Předmět:	Microscopy viruses Electrons Biology Virology Communicable Diseases Virus Microbiology Poliomyelitis virus Tissue culture Microscopy Electron Poliovirus Monkey kidney Particle Poliomyelitis
Zdroj:	Virology. 4(1)
ISSN:	0042-6822
Popis:	A procedure is described for the preparation of freshly harvested infectious tissue culture fluid for quantitative electron microscopy. The yield of characteristic physical particles per monkey kidney epithelial cell varied between 50,000 and 300,000. The average number of physical particles equivalent to one infectious or plaque-forming unit (PFU) was found to be 36 for Mahoney virus. A limited number of experiments on MEF-1 and Saukett viruses indicated particle to PFU ratios of the same order of magnitude for these strains also. Possible reasons for a failure to achieve the ideal ratio of one particle to one PFU have been discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ecfc0738e4262412c0d9a3c860dca0c https://pubmed.ncbi.nlm.nih.gov/13456358 Zobrazit plný text záznamu