Renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmonid fish, detected by nested reverse transcription-PCR of 16S rRNA sequences
| Autor: | Sigridur Gudmundsdottir, E. Benediktsdóttir, Olafur H. Fridjonsson, Ólafur S. Andrésson, Valgerdur Andrésdóttir, H. B. Magnússon |
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| Rok vydání: | 1994 |
| Předmět: |
Molecular Sequence Data
Biology Gram-Positive Bacteria Kidney medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Applied Microbiology and Biotechnology Bacterial genetics law.invention Microbiology Fish Diseases Salmon law RNA Ribosomal 16S medicine Animals Renibacterium salmoninarum Cloning Molecular Gram-Positive Bacterial Infections Polymerase chain reaction Base Sequence Ecology Ovary RNA-Directed DNA Polymerase Ribosomal RNA Hypervariable region Reverse transcription polymerase chain reaction Genes Bacterial Bacterial kidney disease Female Kidney Diseases Nested polymerase chain reaction Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 60:4580-4583 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.60.12.4580-4583.1994 |
| Popis: | An assay based on reverse transcription and nested PCR amplification of hypervariable regions within the 16S rRNA sequence was used to specifically detect Renibacterium salmoninarum, the slowly growing causative agent of bacterial kidney disease in salmonid fish. This assay detected 1 to 10 bacteria per sample and took 1 to 2 days to perform. The assay was used to detect R. salmoninarum in ovarian fluid obtained from naturally infected fish. The assay was unreliable when it was used to examine kidney tissue. |
| Databáze: | OpenAIRE |
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