Separation of alk(en)ylresorcinols from rye bran with saturated, monoenoic, dienoic, trienoic and hydroxylated monoenoic side chains using an octyl phase in ultra-high performance liquid chromatography and their differentiation by tandem mass spectrometrie
| Autor: | Andreas Schieber, Hannes Patzke, Benno F. Zimmermann |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Dietary Fiber
Secale Resolution (mass spectrometry) Hydroxylation Mass spectrometry 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Tandem Mass Spectrometry Acetone Selected ion monitoring Chromatography High Pressure Liquid Chromatography Molecular Structure biology Bran Plant Extracts 010401 analytical chemistry Organic Chemistry Resorcinols 04 agricultural and veterinary sciences General Medicine biology.organism_classification 040401 food science 0104 chemical sciences Triple quadrupole mass spectrometer Molecular Weight chemistry Selectivity |
| Zdroj: | Journal of Chromatography A. 1506:65-72 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2017.05.032 |
| Popis: | Alkylresorcinols (ARs) occur in bran of cereals and in fruits from the Anacardiaceae family. Their separation by liquid chromatography is challenging, especially in rye (Secale cereale L.) that has a complex AR composition. An octyl phase (C8) with 1.8μm particles was used for the analysis of an acetone extract of rye bran. The ARs were detected by UV at 205 and 275nm and by MS applying selected ion monitoring (SIM) of known and hypothetical m/z values in positive and negative mode. The compounds found were subjected to product ion scans in a triple quadrupole mass spectrometer. The C8 UHPLC column has a suitable selectivity for the analysis of ARs from rye. In combination with the sub-2μm particles, baseline separation of most ARs was achieved. The MS2 spectra in positive mode show diagnostic fragments that allow identifying the ARs subclasses (saturated, monoenoic, dienoic, trienoic and hydroxylated monoenoic) unambiguously. Several minor ARs were detected for the first time: C23:3, C27:1OH, C20:0, C22:0, C24:0 and some minor alkenylresorcinol isomers. The chromatographic resolution on the C8 column is unprecedented in the field of rye ARs. Thus, isolation and quantification using non-mass-selective detectors is now possible for each AR. Since rye bran has the most complex AR composition, this method is expected to facilitate the analysis of ARs also in other samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect