Chronic hypertension increases aortic endothelial hydraulic conductivity by upregulating endothelial aquaporin-1 expression
| Autor: | Hadi Fadaifard, George Wolberg, Jimmy Toussaint, Steven Quarfordt, David S. Rumschitzki, Tieuvi Nguyen, Shripad Joshi, Chirag Bharavi Raval, Kung-Ming Jan |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Time Factors Physiology Hydrostatic pressure Rat model 030204 cardiovascular system & hematology Mechanotransduction Cellular Nephrectomy Rats Inbred WKY Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Hydraulic conductivity Rats Inbred SHR Physiology (medical) Internal medicine Cyclic AMP medicine Animals Arterial Pressure Chronic hypertension Aorta Forskolin Aquaporin 1 Models Cardiovascular Anatomy Up-Regulation Disease Models Animal 030104 developmental biology Endocrinology chemistry Chronic Disease Hypertension cardiovascular system Endothelium Vascular Cardiology and Cardiovascular Medicine Research Article |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 313:H1063-H1073 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.00651.2016 |
| Popis: | Numerous studies have examined the role of aquaporins in osmotic water transport in various systems, but virtually none have focused on the role of aquaporin in hydrostatically driven water transport involving mammalian cells save for our laboratory’s recent study of aortic endothelial cells. Here, we investigated aquaporin-1 expression and function in the aortic endothelium in two high-renin rat models of hypertension, the spontaneously hypertensive genetically altered Wistar-Kyoto rat variant and Sprague-Dawley rats made hypertensive by two-kidney, one-clip Goldblatt surgery. We measured aquaporin-1 expression in aortic endothelial cells from whole rat aortas by quantitative immunohistochemistry and function by measuring the pressure-driven hydraulic conductivities of excised rat aortas with both intact and denuded endothelia on the same vessel. We used them to calculate the effective intimal hydraulic conductivity, which is a combination of endothelial and subendothelial components. We observed well-correlated enhancements in aquaporin-1 expression and function in both hypertensive rat models as well as in aortas from normotensive rats whose expression was upregulated by 2 h of forskolin treatment. Upregulated aquaporin-1 expression and function may be a response to hypertension that critically determines conduit artery vessel wall viability and long-term susceptibility to atherosclerosis.NEW & NOTEWORTHY The aortic endothelia of two high-renin hypertensive rat models express greater than two times the aquaporin-1 and, at low pressures, have greater than two times the endothelial hydraulic conductivity of normotensive rats. Data are consistent with theory predicting that higher endothelial aquaporin-1 expression raises the critical pressure for subendothelial intima compression and for artery wall hydraulic conductivity to drop. |
| Databáze: | OpenAIRE |
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