Electrical Impedance Spectroscopy Microflow Cytometer for Cell Viability Tests
| Autor: | Romen Rodriguez-Trujillo, Winnie Edith Svendsen, Albert Romano-Rodriguez, A. Flores, Maria João Moreno, J. Bartoli, J.J. Gonzalez-Murillo, Josep Samitier, M. Monge-Azemar, M. Garcia-Celma |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
010401 analytical chemistry Microfluidics 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Microfluidic channel Electrode Viability assay 0210 nano-technology Electrical impedance spectroscopy Suspension (vehicle) Spectroscopy Electrical impedance Biomedical engineering |
| Zdroj: | 2018 Spanish Conference on Electron Devices (CDE). |
| DOI: | 10.1109/cde.2018.8597037 |
| Popis: | The micro flow cytometer presented here is a combination of a set of coplanar electrodes and a microfluidic channel to test cell viability based on Single-cell Electrical Impedance Spectroscopy analysis. The device was tested both with a control and with a treated HeLa cells suspension. Impedance signals acquired were filtered and processed to obtain the data, classifying and clustering the results by dead and living cells to make the cell viability test. Results were compared with the standards of MTT and Neubauer Chamber viability tests, showing that $0.83\mu \mathbf{L}$ of sample in the prototype designed and fabricated in this work was enough to obtain an approximate response of them. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|