Pseudomonas fildesensis sp. Nov., a psychrotolerant bacterium isolated from antarctic soil of king george island, South Shetland Islands

Autor: Sergio H. Marshall, José L. Martínez, María S. Pavlov, Felipe Lira, Jorge Olivares-Pacheco
Přispěvatelé: Comisión Nacional de Investigación Científica y Tecnológica (Chile), Instituto Antártico Chileno, Pontificia Universidad Católica de Valparaíso (PUCV), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Millennium Nucleus in Plant Cell Biology and Plant Biotechnology Center, Universidad Andrés Bello [Santiago] (UNAB), Instituto Antartico Chileno (INACH) : DT_02-13, Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT), Universidad Tecnica Federico Santa Maria, Centro Nacional de Biotecnologia - Madrid, Spain
Rok vydání: 2020
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
International Journal of Systematic and Evolutionary Microbiology
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2020, 70 (5), pp.3255-3263. ⟨10.1099/ijsem.0.004165⟩
ISSN: 1466-5026
1466-5034
DOI: 10.1099/ijsem.0.004165⟩
Popis: The strain KG01T was isolated from a soil sample from King George Island, Antarctica. Cells of KG01T are rod-shaped and motile by means of multiple polar flagella. The absence of arginine dihydrolase activity could be a key feature to readily distinguish KG01T from its closest phylogenetic relative species. The main fatty acids of the strain include summed feature 3 (C16 : 1 ω7c and/or C15 : 0 iso 2-OH), C16 : 0 and C18 : 1 ω7c. Phylogenetic analysis based on the 16S rRNA gene sequence and on a multilocus sequence analysis (MLSA) using housekeeping genes (16S rRNA, rpoB, rpoD, gyrB) were carried out. These analyses allowed us to include the strain within the Pseudomonas fluorescens group, presenting the highest similarity of multilocus sequence with Pseudomonas veronii LMG 17761T (96.67 %). The genome of KG01T was sequenced and in silico compared with genomes of the most closely related species of the P. fluore scens group. The average nucleotide identity (ANIb) and average amino acid identity (AAI) values of the species phylogenetically closest to KG01T were less than 95–96 %, threshold currently accepted to define strain as belonging to a bacterial species, the highest scores being those to Pseudomonas veronii LMG 17761T (87.98 %) and Pseudomonas marginalis ICMP 3553T (91.90 %). Therefore, the phenotypic and genotypic analyses results, allow us to propose that KG01T represents a member of a novel species of the genus Pseudomonas , for which the name Pseudomonas fildesensis is proposed, and KG01T (=CECT 9084T;=DSM 102036T) is established as the type strain .
This study was supported by the: Soledad S Pavlov , Comisión Nacional de Investigación Científica y Tecnológica , (Award PhD scolarship) Soledad S Pavlov , INACH , (Award DT_02_13)
Databáze: OpenAIRE
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