Increased salt and drought tolerance by D-pinitol production in transgenic Arabidopsis thaliana
| Autor: | Chul-Hyun Ahn, Md. Amir Hossain, Eunjeong Lee, Bashista Kumar Kanth, Phun Bum Park |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Transgene Drought tolerance Biophysics Arabidopsis Genetically modified crops Sodium Chloride Genes Plant Biochemistry 03 medical and health sciences 0302 clinical medicine Gene Expression Regulation Plant Stress Physiological Botany Arabidopsis thaliana Molecular Biology Expressed Sequence Tags biology Abiotic stress Arabidopsis Proteins fungi food and beverages Cell Biology Salt Tolerance biology.organism_classification Plants Genetically Modified Droughts Salinity 030104 developmental biology Seedlings 030220 oncology & carcinogenesis Osmoprotectant Salts Soybeans Inositol |
| Zdroj: | Biochemical and biophysical research communications. 504(1) |
| ISSN: | 1090-2104 |
| Popis: | The methylation of myo-inositol forms O-methyl inositol (d-ononitol) when plants are under abiotic stress in a reaction catalyzed by myo-inositol methyltransferase (IMT). d-Ononitol can serve as an osmoprotectant that prevents water loss in plants. We isolated the IMT cDNA from Glycine max and found by RT-PCR analysis that GmIMT transcripts are induced by drought and salinity stress treatments in the leaves of soybean seedlings. We confirmed the protein product of GmIMT and its substrate using a recombinant system in E. coli. Transgenic Arabidopsis plants over-expressing GmIMT displayed improved tolerance to dehydration stress treatment and to a lesser extent high salinity stress treatment. These results indicate that GmIMT is functional in heterologous Arabidopsis plants. |
| Databáze: | OpenAIRE |
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