Evaluation of systemic inflammatory response and lung injury induced by Crotalus durissus cascavella venom

Autor: Geraldo Pedral Sampaio, Elen Azevedo, Roberto Vieira Pinto, Ilka Biondi, Marcos Lázaro da Silva Guerreiro, Soraya Castro Trindade, Rebeca Pereira Bulhosa Santos, Ricardo Gassmann Figueiredo, Tarsila De Carvalho Freitas Ramos
Rok vydání: 2020
Předmět:
Pathology
Time Factors
Physiology
Toxicology
Pathology and Laboratory Medicine
Biochemistry
Mice
Immune Physiology
Edema
Medicine and Health Sciences
Toxins
Interferon gamma
Snakebite
Immune Response
Materials
Innate Immune System
Multidisciplinary
biology
Ascites
Interleukin
Crotalus
Lung Injury
T-Lymphocytes
Helper-Inducer
Systemic Inflammatory Response Syndrome
Solutions
Physical Sciences
Medicine
Cytokines
Tumor necrosis factor alpha
medicine.symptom
Research Article
Neglected Tropical Diseases
medicine.drug
medicine.medical_specialty
Science
Toxic Agents
Immunology
Materials Science
Inflammation
Gastroenterology and Hepatology
Aqueous Solutions
Lung injury
Signs and Symptoms
Diagnostic Medicine
Crotalid Venoms
medicine
Animals
Envenomation
Venoms
business.industry
Biology and Life Sciences
Proteins
Molecular Development
Tropical Diseases
biology.organism_classification
Immune System
Mixtures
Saline Solutions
business
Collagens
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 2, p e0224584 (2020)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0224584
Popis: This study investigated the systemic inflammatory response and mechanism of pulmonary lesions induced by Crotalus durissus cascavella venom in murine in the state of Bahia. In order to investigate T helper Th1, Th2 and Th17 lymphocyte profiles, we measured interleukin (IL) -2, IL-4, IL-6, IL-10, IL-17, tumor necrosis factor (TNF) and interferon gamma (IFN-γ) levels in the peritoneal fluid and macerated lungs of mice and histopathological alterations at the specific time windows of 1h, 3h, 6h, 12h, 24h and 48h after inoculation with Crotalus durissus cascavella venom. The data demonstrated an increase of acute-phase cytokines (IL-6 and TNF) in the first hours after inoculation, with a subsequent increase in IL-10 and IL-4, suggesting immune response modulation for the Th2 profile. The histopathological analysis showed significant morphological alterations, compatible with acute pulmonary lesions, with polymorphonuclear leukocyte (PMN) infiltration, intra-alveolar edema, congestion, hemorrhage and atelectasis. These findings advance our understanding of the dynamics of envenomation and contribute to improve clinical management and antiophidic therapy for individuals exposed to venom.
Databáze: OpenAIRE
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