Molecular Identification and Amphotericin B Susceptibility Testing of Clinical Isolates of Aspergillus From 11 Hospitals in Korea
| Autor: | Jong Hee Shin, Dong Wook Ryang, Hye Soo Lee, Min Seok Heo, Yeon Joon Park, Won Gil Lee, Sun Hoe Koo, Soo Hyun Kim, Myung Geun Shin, Soon Pal Suh, Min Ji Choi |
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| Rok vydání: | 2015 |
| Předmět: |
Identification
Susceptibility testing Antifungal Agents Clinical Biochemistry Microbial Sensitivity Tests Drug resistance Microbiology Tubulin Amphotericin B Republic of Korea parasitic diseases polycyclic compounds medicine Humans skin and connective tissue diseases DNA Fungal Etest Molecular identification Aspergillus biology Biochemistry (medical) Broth microdilution Sequence Analysis DNA General Medicine Drug susceptibility biochemical phenomena metabolism and nutrition equipment and supplies bacterial infections and mycoses biology.organism_classification Hospitals Clinical Microbiology Mycoses Original Article CLSI medicine.drug |
| Zdroj: | Annals of Laboratory Medicine |
| ISSN: | 2234-3814 2234-3806 |
| DOI: | 10.3343/alm.2015.35.6.602 |
| Popis: | Background We investigated the species distribution and amphotericin B (AMB) susceptibility of Korean clinical Aspergillus isolates by using two Etests and the CLSI broth microdilution method. Methods A total of 136 Aspergillus isolates obtained from 11 university hospitals were identified by sequencing the internal transcribed spacer (ITS) and β-tubulin genomic regions. Minimal inhibitory concentrations (MICs) of AMB were determined in Etests using Mueller-Hinton agar (Etest-MH) and RPMI agar (Etest-RPG), and categorical agreement with the CLSI method was assessed by using epidemiological cutoff values. Results ITS sequencing identified the following six Aspergillus species complexes: Aspergillus fumigatus (42.6% of the isolates), A. niger (23.5%), A. flavus (17.6%), A. terreus (11.0%), A. versicolor (4.4%), and A. ustus (0.7%). Cryptic species identifiable by β-tubulin sequencing accounted for 25.7% (35/136) of the isolates. Of all 136 isolates, 36 (26.5%) had AMB MICs of ≥2 µg/mL by the CLSI method. The categorical agreement of Etest-RPG with the CLSI method was 98% for the A. fumigatus, A. niger, and A. versicolor complexes, 87% for the A. terreus complex, and 37.5% for the A. flavus complex. That of Etest-MH was ≤75% for the A. niger, A. flavus, A. terreus, and A. versicolor complexes but was higher for the A. fumigatus complex (98.3%). Conclusions Aspergillus species other than A. fumigatus constitute about 60% of clinical Aspergillus isolates, and reduced AMB susceptibility is common among clinical isolates of Aspergillus in Korea. Molecular identification and AMB susceptibility testing by Etest-RPG may be useful for characterizing Aspergillus isolates of clinical relevance. |
| Databáze: | OpenAIRE |
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