Production of a Type 2 Maternal Diabetes Rodent Model Using the Combination of High-Fat Diet and Moderate Dose of Streptozocin
| Autor: | Steven D. Holladay, M. Renee Prater, Claudia P Yefi, Javiera Bahamonde, J. Claudio Gutierrez |
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| Rok vydání: | 2010 |
| Předmět: |
Blood Glucose
medicine.medical_specialty endocrine system diseases medicine.medical_treatment Pregnancy in Diabetics Blood sugar Maternal diabetes Type 2 diabetes Streptozocin Mice Endocrinology Pregnancy Internal medicine Diabetes mellitus medicine Animals Insulin Type 1 diabetes business.industry Body Weight nutritional and metabolic diseases General Medicine medicine.disease Dietary Fats Diabetes Gestational Disease Models Animal Diabetes Mellitus Type 2 Female business |
| Zdroj: | Endocrine Research. 35:59-70 |
| ISSN: | 1532-4206 0743-5800 |
| DOI: | 10.3109/07435801003641939 |
| Popis: | Pregnancy may be complicated by maternal diabetes. The following experiments were performed in an attempt to produce mouse models of insulin-resistant maternal diabetes.CD1 females received 200 mg/kg streptozocin (STZ) to model insulin-dependent diabetes (T1 group). Another group of females (T2 group) was put on a HFD 4 weeks before receiving 100 mg/kg STZ. After 4 additional weeks of HFD, hyperglycemic females were separated and bred. In another experiement, CD1 females were fed a HFD for 4 weeks before receiving an intravenous (GDM1 group) or intraperitoneal (GDM2 group) injection of 100 mg/kg STZ. Females from GDM2 group were bred at the same day of the STZ injection. Females from GDM1 group were bred 4 weeks after the STZ injection.About 25% of the females from T2 group became hyperglycemic after 4 weeks of the injection of STZ. Fifty percent of the females from GDM1 group reached hyperglycemic levels greater than 250 mg/dl during pregnancy. The combination of HFD and moderate STZ in CD1 mice therefore produced hyperglycemic females; however numbers of these mice were somewhat low. |
| Databáze: | OpenAIRE |
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