Antibiotic Resistance in Primary and Persistent Endodontic Infections
| Autor: | M. Tolba, Renu Nandakumar, Ashraf F. Fouad, Richard A. Venezia, Gretchen B. Jungermann, Krystal Burns |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
DNA Bacterial medicine.drug_class medicine.medical_treatment Antibiotics Microbial Sensitivity Tests Drug resistance Biology Article beta-Lactam Resistance Microbiology Bacterial genetics law.invention Young Adult Antibiotic resistance law Drug Resistance Bacterial medicine Humans General Dentistry Polymerase chain reaction Aged Aged 80 and over Periapical periodontitis Root Canal Irrigants Dental Pulp Diseases Tetracycline Resistance Vancomycin Resistance Middle Aged biochemical phenomena metabolism and nutrition medicine.disease biology.organism_classification Genes Bacterial Acute Disease Chronic Disease Beta-lactamase Periapical Periodontitis Root Canal Preparation Bacteria |
| Zdroj: | Journal of Endodontics. 37:1337-1344 |
| ISSN: | 0099-2399 |
| DOI: | 10.1016/j.joen.2011.06.028 |
| Popis: | Introduction The presence of antibiotic resistance genes in endodontic microorganisms might render the infection resistant to common antibiotics. The aims of this project were to identify selected antibiotic resistance genes in primary and persistent endodontic infections and to determine the effectiveness of contemporary endodontic procedures in eliminating bacteria with these genes. Methods In patients undergoing primary endodontic treatment or retreatment, the root canals were aseptically accessed and sampled before endodontic procedures as well as after contemporary chemomechanical preparation and medication with calcium hydroxide. Identification of the following antibiotic resistance genes was performed by using polymerase chain reaction: bla TEM-1 , cfx A, bla Z, tet M, tet W, tet Q, van A, van D, and van E. Limited phenotypic identification and antibiotic susceptibility verification were also performed. Results Overall, there were 45 specimens available for analysis, 30 from primary and 15 from persistent endodontic infections. In preoperative specimens, only bla TEM-1 was significantly more prevalent in primary versus persistent infections ( P = .04). After contemporary treatment procedures, there was an overall reduction in prevalence of these genes ( P bla TEM-1 and tet W were significantly reduced ( P cfx A, bla Z, and tet Q were eliminated, but there was no change in tet M. No specimens contained vanA , vanD , or vanE . Antibiotic susceptibility testing showed significant differences among the antibiotics ( P Conclusions bla TEM -1 was more prevalent in primary than persistent infections. Vancomycin resistance was not present. The genes identified were reduced with treatment except for tet M. Genetic testing might be useful as a screening tool for antibiotic resistance. |
| Databáze: | OpenAIRE |
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