Bovine Leukemia Virus High Tax Molecular Clone Experimentally Induces Leukemia/Lymphoma in Sheep
| Autor: | Satoru Konnai, Shigeru Numakunai, Manabu Ikeda, Masanobu Goryo, Shan-ai Yin, Tou-ichi Hirata, Misao Onuma, Masahiko Takahashi, Konomi Kuramochi, Shigeru Tajima, Shin-nosuke Takeshima, Yoko Aida, Norihiro Nakae, Shigeaki Takami, Kosuke Okada |
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| Rok vydání: | 2005 |
| Předmět: |
Clone (cell biology)
Sheep Diseases Immunoglobulin light chain Immunophenotyping hemic and lymphatic diseases Leukemia Virus Bovine medicine Animals Leukemia-Lymphoma Adult T-Cell Glycoproteins MHC class II Sheep General Veterinary Bovine leukemia virus biology Myocardium Gene Products tax Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease biology.organism_classification Immunohistochemistry Virology Lymphoma Gastrointestinal Tract Leukemia biology.protein Lymph Nodes Lymph CD5 |
| Zdroj: | Journal of Veterinary Medical Science. 67:1231-1235 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.67.1231 |
| Popis: | Sheep were inoculated with high tax coded pBLV-IF (H group, Nos.1-5) of bovine leukemia virus (BLV), wild tax coded pBLV-IF (W group, Nos. 6-11), or control plasmid (C group, Nos. 12-14). During the observation period (4 to 46 months), 5 of 5 cases in H group and 3 of 6 cases (Nos. 6, 7, 9) in W group became positive for gp 51. Only 1 case in H group became leukemic, and one case each of H and W groups developed lymphoma. In No. 3, lesions were found in multiple organs including the lymph nodes, gastrointestinal tract following abomasum, and heart. In No. 6, lesions of lymphoma were found only in the jejunum and heart. Morphologically, small to middle-sized lymphocytic neoplastic (NP) cells were found in both cases, but lymphoblastic NP cells were found only in No. 3. By immunohistochemical examination, the phenotypes of NP cells were determined as CD1 - , CD4 - , CD5 - -, CD8α - , sIgM + , λ light chain + , B-B4 + , MHC class II + in both case. The results of this study indicate that inoculation of pBLV-IF can induce lymphocytic and lymphoblastic leukemia/lymphoma in sheep. Additionally, it is suggested that the expression rate of tax gene is not associated with the development of leukemia/lymphoma in sheep experimentally inoculated with pBLV-IF. |
| Databáze: | OpenAIRE |
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