Application of a multiplex polymerase chain reaction (mPCR) assay to detect fraud by substitution of bovine meat cuts with water buffalo meat in Northern Brazil
| Autor: | Carina Martins de Moraes, Wanessa Shuelen Costa Araújo, Andrey Carlos do Sacramento de Oliveira, Talita Bandeira Roos, Andréia Silva da Silva, Josyane Brasil da Silva, Lúcia de Fátima Henriques Lourenço, Silvia Cristina da Silva Pedroso, Gabrielle Virgínia Ferreira Cardoso, Vanderson Vasconcelos Dantas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
food fraud lcsh:TP368-456 030309 nutrition & dietetics General Chemical Engineering Substitution (logic) species identification food and beverages multiplex pcr lcsh:TX341-641 04 agricultural and veterinary sciences General Chemistry dna extraction 040401 food science Industrial and Manufacturing Engineering 03 medical and health sciences lcsh:Food processing and manufacture 0404 agricultural biotechnology Water buffalo Multiplex polymerase chain reaction Food science Product (category theory) Business lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | CyTA-Journal of Food, Vol 17, Iss 1, Pp 790-795 (2019) |
| ISSN: | 1947-6345 1947-6337 |
| Popis: | The adulteration of meat products can affect the confidence of consumers and the market, leading to negative impacts on the economy. Accordingly, product authenticity has become an important issue in modern society. Therefore, our study aimed to optimize the extraction of DNA from meat and use multiplex polymerase chain reaction (mPCR) to determine the incidence of fraud by substitution of bovine meat cuts with water buffalo meat in the states of Pará and Amapá, Northern Brazil. The mPCR protocol used primers that amplify sequences of 346 base pairs of bovine DNA and 220 base pairs of buffalo DNA. To assess the sensitivity of the technique, a standardized PCR assay was performed using the template DNA extracted and diluted from 10−0 to 10−10 in PCR-grade water. Next, 161 samples of meat cuts marketed as bovine (rump) origin were collected in the states of Pará and Amapá, Northern Brazil. The mPCR assays demonstrated good specificity of the primers used. The sensitivity test amplified bovine and buffalo DNA fragments down to the 10−2 dilution. The results demonstrated fraud by substitution of beef by water buffalo meat in 21.7% of samples, demonstrating that this act does occur intentionally for economic gains. |
| Databáze: | OpenAIRE |
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