Protein-protein interaction between ezrin and p65 in human breast cancer cells
| Autor: | Xiao-Ran Yu, Fu-Xi Li, R Tang, J B Xiong, Wen-Feng Shao, Q S Wen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Breast Neoplasms Endogeny macromolecular substances Plasma protein binding environment and public health Protein–protein interaction 03 medical and health sciences 0302 clinical medicine Ezrin Cell Line Tumor Genetics Humans skin and connective tissue diseases Molecular Biology Chemistry Novel protein Transcription Factor RelA General Medicine Cytoskeletal Proteins 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Cancer cell Cancer research Human breast Protein Binding |
| Zdroj: | Genetics and Molecular Research. 15 |
| ISSN: | 1676-5680 |
| DOI: | 10.4238/gmr.15028334 |
| Popis: | Our study aimed to investigate the co-localization and protein-protein interactions between ezrin and p65 in human breast cancer cells. Liquid chromatography-mass spectrometry (LCMS) was used to uncover novel protein interactions with ezrin in MDA-MB-231 cells. Endogenous co-immunoprecipitation was used to validate protein-protein interactions between ezrin and p65 in MDA-MB-231. Exogenous interactions between ezrin and p65 were validated in MDA-MB-231 cells via Flag-ezrin and HA-p65 co-transfection and followed by co-immunoprecipitation. Immunofluorescence staining was used to visualize ezrin and p65 co-localization in MDA-MB-231. LCMS results showed that there were 1000 proteins interacting with ezrin in MDA-MB-231 cells. Ezrin and p65 interactions were confirmed with both endogenous and exogenous methods. We were also able to visualize ezrin and p65 co-localization in MDA-MB-231. In summary, we found protein-protein interactions between Ezrin and p65 in human breast cancer cells. |
| Databáze: | OpenAIRE |
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