Size does matter: Cre-mediated somatic deletion efficiency depends on the distance between the target lox-sites
| Autor: | Femke van Gennip, Mark J.J. van Haaren, Bart J. M. Hersmus, Eric R. Coppoolse, Marianne J. de Vroomen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
DNA
Plant Somatic cell Bioinformatics transgenic tomato plants gene cloning Plant Science Molecular cloning Biology dna Chromosomes Plant Transposition (music) chemistry.chemical_compound Viral Proteins Plasmid Solanum lycopersicum expression Bioinformatica Genetics transposition Site-specific recombination Gene genome Crosses Genetic Recombination Genetic Binding Sites Integrases Chromosome Mapping food and beverages General Medicine Molecular biology recombination chromosome rearrangements chemistry DNA Transposable Elements Nucleic Acid Conformation identification Chromosome Deletion EPS Agronomy and Crop Science Recombination DNA biotechnology |
| Zdroj: | Plant Molecular Biology, 58(5), 687-698 Plant Molecular Biology 58 (2005) 5 |
| ISSN: | 0167-4412 |
| Popis: | Cre/lox recombination in vivo has become an important tool to induce chromosomal rearrangements like deletions. Using a combination of Ds transposition and Cre/lox recombination in two independent experiments on chromosomes 6 and 7 of tomato, two sets of somatic deletions up to a size of 200 kb were obtained. The efficiency of somatic deletion decreased with increasing deletion size. The largest germinally transmitted deletion had a size of only 55 kb. The results show that Cre-mediated deletion in somatic cells is less efficient when the lox sites are separated over larger distances. A further drop of the deletion efficiency after germinal transmission of the larger deletions can be explained by the probable loss of genes that are of vital importance to gametophyte function. Plasmid rescue of an 8.4 kb circularised deleted DNA showed that the Cre-mediated deletion takes place in tomato as expected. Since the circular Cre-deleted DNA could only be PCR amplified in plant cells where the deletion was not complete, the double-stranded DNA circle is assumed to be instable. |
| Databáze: | OpenAIRE |
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