TSGP4 from Ornithodoros moubata: molecular cloning, phylogenetic analysis and vaccine efficacy of a new member of the lipocalin clade of cysteinyl leukotriene scavengers
| Autor: | Raúl Manzano-Román, Prosper Obolo-Mvoulouga, Verónica Díaz-Martín, Ricardo Pérez-Sánchez, Álvaro Oleaga |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Ministerio de Economía y Competitividad (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Models
Molecular 0301 basic medicine Protein Conformation Lipocalin Molecular cloning Antibodies Recombinant antigens Arthropod Proteins 03 medical and health sciences Immune system Antigen Ornithodoros moubata TSGP4 Animals Amino Acid Sequence Antigens Cloning Molecular Ornithodoros Phylogeny Vaccines General Veterinary biology General Medicine biology.organism_classification Vaccine efficacy Virology Tick Infestations Vaccination 030104 developmental biology Gene Expression Regulation biology.protein Parasitology Rabbits Antibody |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 24 páginas. -- The definitive vesion is available at http://www.elsevier.com Recently obtained evidence indicated that an orthologue of the O. savignyi TSGP4 salivary lipocalin was present in the saliva of O. moubata. TSGP4 is known to act as a cysteinyl leukotrienes scavenger helping in the prevention of inflammation and oedema at the tick bite site. Since this function seems to be crucial for successful tick feeding, the novel O. moubata TSGP4 turned into a potential vaccine target. The purposes of the current work were: (i) to clone and characterize the O. moubata TSGP4 and, (ii) to produce it as recombinant to evaluate its protective efficacy as vaccine antigen. The results of these experiments indicated that the O. moubata TSGP4 shows high sequence and structural identity with the O. savignyi orthologue suggesting identical function in the physiology of the tick-host relationship. The mature native TSGP4 is not immunogenic when it is inoculated to host with tick saliva during feeding, but host vaccination with the recombinant protein TSGP4 in Freund’s adjuvants induced strong humoral immune responses that recognized both the recombinant and native TSGP4 and protected the host with a 14.1% efficacy. So, the O. moubata TSGP4 can be considered a silent salivary antigen; however, in the light of the current results, its inclusion in the current repertory of protective antigens to be targeted by anti-tick vaccines could be controversial. Financial support provided by the Spanish Ministry of Science and Innovation (Grant no. AGL2010-18164) and Ministry of Economy and Competitivity (Grant no. AGL2013-42745-P). |
| Databáze: | OpenAIRE |
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