Antineoplastic Effects of N6-(△2-Isopentenyl)adenosine against L-1210 Mouse Lymphocytic Leukemic Cells Using a Polymeric Delivery System
| Autor: | Yunik Chang, Bruce Hacker |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Drug
Adenosine Polymers Chemistry Stereochemistry media_common.quotation_subject Pharmaceutical Science Antineoplastic Agents Cell cycle Loading dose In vitro Isopentenyladenosine Mice medicine Biophysics Animals Cytotoxic T cell Leukemia L1210 Nucleoside Cells Cultured Chromatography High Pressure Liquid ED50 medicine.drug media_common |
| Zdroj: | Journal of Pharmaceutical Sciences. 71:328-331 |
| ISSN: | 0022-3549 |
| DOI: | 10.1002/jps.2600710317 |
| Popis: | N 6 -(△ 2 -Isopentenyl)adenosine (I), a nucleoside previously shown to be cytotoxic against several types of tumor cells, was impregnated in silicone polymer monolithic disc devices for release in vitro against lymphocytic mouse leukemia cells. Plotting the cumulative amount of N 6 -(△ 2 -isopentenyl)adenosine released per unit area of the device versus the square root of time revealed a linear relationship. However, the higher loading dose tended to rapidly release any drug deposited on the polymer surface. The optimum loading dose of the device for the most effective antileukemic activity in 24 hr was calculated based on a plot of the release rate versus the square root of an initial loading dose. The silicone polymer-I delivery system enabled a sustained and controllable release of additional agent. It was thus possible to achieve virtually total inhibition of leukemic cell replication using the polymeric delivery system. Increased concentrations of I, without the use of the polymeric system, resulted in maximum 24 hr inhibition of only ~ 81%, followed by a decline in overall antileukemic activity. It is possible to achieve a more predictable release rate of N 6 -(△ 2 -isopentenyl)aden-osine and corresponding antileukemic activity using a polymeric delivery system against L-1210 mouse leukemic cells in vitro. The relative data indicate the ED50 concentrations to be considerably less using the polymeric delivery system. |
| Databáze: | OpenAIRE |
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