Fast digestive, leucine-rich, soluble milk proteins improve muscle protein anabolism and mitochondrial function in undernourished old rats

Autor: Audrey Chanet, Alexandre Berry, Philippe Denis, Yves Boirie, Corinne Pouyet, Yves Rolland, Christophe Rocher, Carla Domingues-Faria, Stéphane Walrand, Jérôme Salles, Christophe Giraudet, Christelle Guillet, Pascale Le Ruyet, Cécile Bonhomme, Véronique Patrac
Přispěvatelé: Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB), Groupe Lactalis, Institut du Vieillissement, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Epidémiologie et analyses en santé publique : risques, maladies chroniques et handicaps (LEASP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Clinical Nutrition Department, CHU Clermont-Ferrand, French National Agency for Research (ANR), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire de Clermont-Ferrand, Université de Toulouse (UT)-Université de Toulouse (UT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Toulouse (UT)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
old rats
Anabolism
[SDV]Life Sciences [q-bio]
Muscle Proteins
Muscle Development
rat agé
anabolisme
Tripartite Motif Proteins
Random Allocation
protéine de lait
Casein
Whole Body Imaging
soluble milk proteins
senior citizens
2. Zero hunger
Chemistry
anabolism
protéine soluble
nutritional deficiency
Milk Proteins
Magnetic Resonance Imaging
medicine.anatomical_structure
Biochemistry
Elder Nutritional Physiological Phenomena
Alimentation et Nutrition
Digestion
Leucine
Biotechnology
medicine.medical_specialty
métabolisme énergétique
Ubiquitin-Protein Ligases
malnutrition
personne âgée
03 medical and health sciences
Internal medicine
medicine
Food and Nutrition
Animals
refeeding
skeletal muscle
Rats
Wistar
Muscle
Skeletal
030109 nutrition & dietetics
SKP Cullin F-Box Protein Ligases
Skeletal muscle
Metabolism
protéine musculaire
medicine.disease
Mitochondria
Muscle
Malnutrition
030104 developmental biology
Endocrinology
Solubility
Dietary Supplements
Proteolysis
Lean body mass
Energy Metabolism
Protein quality
Biomarkers
Food Science
Zdroj: Molecular Nutrition and Food Research
Molecular Nutrition and Food Research, Wiley-VCH Verlag, 2017, 61 (11), ⟨10.1002/mnfr.201700287⟩
Molecular Nutrition and Food Research, Wiley-VCH Verlag, 2017, ⟨10.1002/mnfr.201700287⟩
Molecular Nutrition and Food Research, 2017, 61 (11), ⟨10.1002/mnfr.201700287⟩
Molecular Nutrition and Food Research 11 (61), 13 p.. (2017)
ISSN: 1613-4125
1613-4133
Popis: in press; SCOPE: One strategy to manage malnutrition in older patients is to increase protein and energy intake. Here we evaluate the influence of protein quality during refeeding on improvement in muscle protein and energy metabolism.METHODS AND RESULTS: Twenty-month-old male rats (n = 40) were fed 50% of their spontaneous intake for 12 weeks to induce malnutrition, then refed ad libitum with a standard diet enriched with casein or soluble milk proteins (22%) for 4 weeks. 13C-valine was infused to measure muscle protein synthesis and expression of MuRF1, and MAFbx was measured to evaluate muscle proteolysis. mTOR pathway activation and mitochondrial function were assessed in muscle. Malnutrition was associated with a decrease in body weight, fat mass and lean mass, particularly muscle mass. Malnutrition decreased muscle mTOR pathway activation and protein FSR associated with increased MuRF1 mRNA levels, and decreased mitochondrial function. The refeeding period partially restored fat mass and lean mass. Unlike the casein diet, the soluble milk protein diet improved muscle protein metabolism and mitochondrial function in old malnourished rats.CONCLUSIONS: These results suggest that providing better-quality proteins during refeeding may improve efficacy of renutrition in malnourished older patients. This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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