Duration of protection and humoral immunity induced by an adenovirus-vectored subunit vaccine for foot-and-mouth disease (FMD) in Holstein steers
| Autor: | Juan M. Pacheco, William T. Golde, Tatjana Sitt, Mital Pandya, Korin Eckstrom, Jonathan Arzt, Megan Warner, Michael LaRocco, David A. Brake, Elizabeth Rieder, Javier Palarea-Albaladejo, José Barrera, John W. Barlow, Mary Kenney |
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| Rok vydání: | 2019 |
| Předmět: |
viruses
030231 tropical medicine Genetic Vectors Cattle Diseases Antibodies Viral Viral vector 03 medical and health sciences 0302 clinical medicine Immunity Medicine Animals 030212 general & internal medicine Viral shedding Vaccines Synthetic General Veterinary General Immunology and Microbiology biology Foot-and-mouth disease business.industry Adenoviruses Human Vaccination Public Health Environmental and Occupational Health Viral Vaccines biology.organism_classification medicine.disease Virology Antibodies Neutralizing Immunity Humoral Infectious Diseases Foot-and-Mouth Disease Virus Foot-and-Mouth Disease Humoral immunity Vaccines Subunit biology.protein Molecular Medicine Capsid Proteins Cattle Antibody Foot-and-mouth disease virus business |
| Zdroj: | Vaccine. 37(42) |
| ISSN: | 1873-2518 |
| Popis: | Foot-and-mouth disease (FMD) is a highly contagious viral infection of cloven hooved animals that continues to cause economic disruption in both endemic countries or when introduced into a formally FMD free country. Vaccines that protect against clinical disease and virus shedding are critical to control FMD. The replication deficient human adenovirus serotype 5 (Ad5) vaccine vector expressing empty FMD virus (FMDV) capsid, AdtFMD, is a promising new vaccine platform. With no shedding or spreading of viral vector detected in field trials, this vaccine is very safe to manufacture, as there is no requirement for high containment faciitites. Here, we describe three studies assessing the proportion of animals protected from clinical vesicular disease (foot lesions) following live-FMDV challenge by intradermolingual inoculation at 6 or 9 months following a single vaccination with the commercial AdtFMD vaccine, provisionally licensed for cattle in the United States. Further, we tested the effect of vaccination route (transdermal, intramuscular, subcutaneous) on clinical outcome and humoral immunity. Results demonstrate that a single dose vaccination in cattle with the commercial vaccine vector expressing capsid proteins of the FMDV strain A24 Cruzeiro (Adt.A24), induced protection against clinical FMD at 6 months (100% transdermal, 80% intramuscular, and 60% subcutaneous) that waned by 9 months post-vaccination (33% transdermal and 20% intramuscular). Post-vaccination serum from immunized cattle (all studies) generally contained FMDV specific neutralizing antibodies by day 14. Anti-FMDV antibody secreting cells are detected in peripheral blood early following vaccination, but are absent after 28 days post-vaccination. Thus, the decay in antibody mediated immunity over time is likely a function of FMDV-specific antibody half-life. These data reveal the short time span of anti-FMDV antibody secreting cells (ASCs) and important performance characteristics of needle-free vaccination with a recombinant vectored subunit vaccine for FMDV. |
| Databáze: | OpenAIRE |
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