The use of native fluorescence analysis of synovial fluid in the diagnosis of medial compartment disease in medium- and large-breed dogs
| Autor: | Mária Mareková, Valent Ledecký, Kamila Bilská, Marián Hluchý, Zuzana Šteffeková, Anna Birková, Terézia Kisková |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty 040301 veterinary sciences Osteoarthritis Sensitivity and Specificity 0403 veterinary science 03 medical and health sciences Dogs Elbow Joint Synovial Fluid medicine Animals Synovial fluid Dog Diseases Compartment (pharmacokinetics) General Veterinary Chemistry Area under the curve 04 agricultural and veterinary sciences medicine.disease Fluorescence Breed Autofluorescence Fluorescence intensity Spectrometry Fluorescence 030104 developmental biology Case-Control Studies Female |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 28:332-337 |
| ISSN: | 1943-4936 1040-6387 |
| Popis: | We assumed that proteins are most likely responsible for synovial fluid fluorescence and that changes detected in fluorescence intensity are most likely the result of changes in the concentration of fluorescent proteins. Synchronous fluorescent matrices from synovial fluid samples were measured in the excitation wavelength range of 200–350 nm using a luminescence spectrophotometer. The synchronous matrix of synovial fluid consists of 2 dominant fluorescent centers (F1 and F2) in the ultraviolet region. The fluorescence intensities of both centers were significantly higher in pathological samples, with p = 0.001 (a 59% increase of the median value) for the F1 center and p = 0.002 (a 52% increase of the median value) for the F2 center. Receiver operating characteristic analysis confirmed that synovial fluid autofluorescence is a significant predictor of medial compartment disease in dogs, with the area under the curve at 0.776 (F1) and 0.778 (F2). We did not detect any differences in the autofluorescence of synovial fluid between male and female, or any breed-based changes. No position changes of fluorescent centers were recorded in the synovial fluid in diseased dogs compared with healthy dogs. The synovial fluid metabolic fingerprint of canine patients with medial compartment disease differed from that of healthy dogs. Our study demonstrated the feasibility of synovial fluid fingerprinting to identify disease-specific profiles of synovial fluid metabolites. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|