Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco
| Autor: | Jussi Joensuu, Bert Devriendt, Angelo Kaldis, Rima Menassa, Eric Cox, Igor Kolotilin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
PROTEIN EXPRESSION
Swine HIGH-LEVEL EXPRESSION Agricultural Biotechnology F4 K88 FIMBRIAE Fimbria Gene Expression Plant Science medicine.disease_cause Bacterial Adhesion Enterotoxigenic Escherichia coli NUCLEOTIDE-SEQUENCE Biomass Plastids Swine Diseases Adhesins Escherichia coli Multidisciplinary biology Microvilli Protein Stability Genetically Modified Organisms Vaccination Agriculture Plants Genetically Modified Veterinary Bacteriology Recombinant Proteins CHLOROPLAST TRANSFORMATION Diarrhea Phenotype Veterinary Diseases Vaccines Subunit Medicine PSBA MESSENGER-RNA medicine.symptom Genetic Engineering ORAL IMMUNIZATION Research Article Biotechnology Protein subunit Science Animal Types Weaning Large Animals Microbiology Transformation Genetic SDG 3 - Good Health and Well-being Tobacco Vaccine Development medicine Genetics Animals Veterinary Sciences Biology ENTEROTOXIGENIC ESCHERICHIA-COLI Nicotiana MAJOR SUBUNIT Wild type Immunity biology.organism_classification Virology TRANSGENIC TOBACCO Gastrointestinal Tract Fimbriae Bacterial Plant Biotechnology Clinical Immunology Veterinary Science Transplastomic plant Transgenics |
| Zdroj: | PLOS ONE PLoS ONE Kolotilin, I, Kaldis, A, Devriendt, B, Joensuu, J, Cox, E & Menassa, R 2012, ' Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco ', PLoS ONE, vol. 7, no. 8, 42405 . https://doi.org/10.1371/journal.pone.0042405 PLoS ONE, Vol 7, Iss 8, p e42405 (2012) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0042405 |
| Popis: | Post-weaning diarrhea (PWD) in piglets is a major problem in piggeries worldwide and results in severe economic losses. Infection with Enterotoxigenic Escherichia coli (ETEC) is the key culprit for the PWD disease. F4 fimbriae of ETEC are highly stable proteinaceous polymers, mainly composed of the major structural subunit FaeG, with a capacity to evoke mucosal immune responses, thus demonstrating a potential to act as an oral vaccine against ETEC-induced porcine PWD. In this study we used a transplastomic approach in tobacco to produce a recombinant variant of the FaeG protein, rFaeG(ntd/dsc), engineered for expression as a stable monomer by N-terminal deletion and donor strand-complementation (ntd/dsc). The generated transplastomic tobacco plants accumulated up to 2.0 g rFaeG(ntd/dsc) per 1 kg fresh leaf tissue (more than 1% of dry leaf tissue) and showed normal phenotype indistinguishable from wild type untransformed plants. We determined that chloroplast-produced rFaeG(ntd/dsc) protein retained the key properties of an oral vaccine, i.e. binding to porcine intestinal F4 receptors (F4R), and inhibition of the F4-possessing (F4+) ETEC attachment to F4R. Additionally, the plant biomass matrix was shown to delay degradation of the chloroplast-produced rFaeG(ntd/dsc) in gastrointestinal conditions, demonstrating a potential to function as a shelter-vehicle for vaccine delivery. These results suggest that transplastomic plants expressing the rFaeG(ntd/dsc) protein could be used for production and, possibly, delivery of an oral vaccine against porcine F4+ ETEC infections. Our findings therefore present a feasible approach for developing an oral vaccination strategy against porcine PWD. |
| Databáze: | OpenAIRE |
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