Characterization of livestock-associated methicillin-resistant 'Staphylococcus aureus' isolates obtained from commercial rabbitries located in the Iberian Peninsula
| Autor: | Asunción Muñoz-Silvestre, Celia Sanz-Tejero, L. Selva, Ana B. Fernández-Ros, Sara Pérez-Fuentes, Elena Moreno-Grua, D. Viana, J.M. Corpa |
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| Přispěvatelé: | UCH. Departamento de Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Producción Científica UCH 2018 |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Staphylococcus aureus 030106 microbiology rabbit lcsh:QR1-502 Virulence MRSA Biology medicine.disease_cause Microbiology lesions lcsh:Microbiology 03 medical and health sciences Staphylococcus - Drug resistance Genotype LA-MRSA medicine Rabbits - Diseases Typing Conejos - Enfermedades SCCmec Phenotypic trait biochemical phenomena metabolism and nutrition bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus 030104 developmental biology Multilocus sequence typing Estafilococos - Resistencia a los medicamentos |
| Zdroj: | Frontiers in Microbiology, Vol 9 (2018) CEU Repositorio Institucional Fundación Universitaria San Pablo CEU (FUSPCEU) |
| Popis: | Este artículo se encuentra disponible en la página web de la revista en la siguiente URL: https://www.frontiersin.org/articles/10.3389/fmicb.2018.01812/full Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have been a growing problem in human medicine since the 1960s, and more recently in veterinary medicine with the appearance of livestock-associated MRSA (LA-MRSA). Nevertheless, information about the presence of MRSA in rabbits is quite scarce since only one LA-MRSA identification has been previously reported. The present study aimed to determine genotypic characterization by verifying the presence of resistance determinants, virulence, and toxin genes of different S. aureus strains that cause lesions in rabbits, and their phenotypic traits based on the antimicrobial susceptibility profile. The analysis of 240 S. aureus isolates obtained from different lesion types collected from 89 Spanish and Portuguese rabbit commercial farms in the last 4 years (2014–2017) was performed. The methicillin-resistant gene mecA was found in 11.25% of the studied isolates (27 of 240) from 19 farms (13 Spanish and 6 Portuguese). Staphylococcal cassette chromosome mec (SCCmec) typing predominantly revealed type III (n = 15). Additionally, three MRSA isolates carrying the mecC gen were detected in samples from three different farms (two Spanish and one Portuguese). None of the 30 MRSA isolates was PVL-positive or tst-positive. After the multilocus sequence typing (MLST) procedure, 16 belonged to ST2855, 6 to ST146, 6 to ST398, and 2 ST4774. No ST121 isolate was mec-positive. ST398 and ST4774 isolates lacked the immuneevasion-cluster (IEC) genes. ST2855 strains were associated with the presence only of the sak gene, and ST146 isolates were ascribed to IEC type E. Therefore, this is the first description of LA-MRSA from rabbits belonging to ST2855. Interestingly, one ST2855 and two ST4774 isolates were mecC-positive, which could act as a mecCMRSA reservoir. More studies are needed to further characterize these isolates and their relationship with humans and other animal species. |
| Databáze: | OpenAIRE |
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