Morphological heterogeneity ofParacoccidioides brasiliensis: relevance of the Rho-like GTPasePbCDC42
| Autor: | Nuno S. Osório, Fernando Rodrigues, Paula Ludovico, Ana G. Gomes-Alves, Mark H. J. Sturme, Agostinho J. Almeida, João F. Menino, Patrício Costa, Gustavo H. Goldman, Diana Barros |
|---|---|
| Přispěvatelé: | Faculdade de Psicologia e de Ciências da Educação, Universidade do Minho |
| Rok vydání: | 2012 |
| Předmět: |
Morphology
FUNGOS MITOSPORICOS Ciências veterinárias Cell PbCDC42 Fungus GTPase Biology Pathogenesis Veterinary science 03 medical and health sciences Veterinary science [Agrarian Sciences] Botany Environmental Microbiology medicine Humans Ciências veterinárias [Ciências agrárias] 030304 developmental biology cdc42 GTP-Binding Protein Saccharomyces cerevisiae Paracoccidioides brasiliensis Genetics Microscopy 0303 health sciences Budding Polymorphism Genetic Science & Technology Phylogenetic tree Strain (chemistry) 030306 microbiology Paracoccidioides General Medicine biology.organism_classification 3. Good health Infectious Diseases medicine.anatomical_structure Gene Knockdown Techniques Paracoccidioidomycosis Predictor |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1460-2709 1369-3786 |
| Popis: | We thank Rosana Puccia for providing isolate Pb02. Paracoccidioides brasiliensis budding pattern and polymorphic growth were previously shown to be closely linked to the expression of PbCDC42 and to infl uence the pathogenesis of the fungus. In this work we conducted a detailed morphogenetic evaluation of the yeast-forms of 11 different clinical and environmental P. brasiliensis isolates comprising four phylogenetic lineages (S1, PS2, PS3 and Pb01-like), as well as a PbCDC42 knockdown strain. High variations in the shape and size of mother and bud cells of each isolate were observed but we did not fi nd a characteristic morphologic profi le for any of the phylogenetic groups. In all isolates studied, the bud size and shape were demonstrated to be highly dependent on the mother cell. Importantly, we found strong correlations between PbCDC42 expression and both the shape of mother and bud cells and the size of the buds in all isolates and the knock-down strain. Our results suggested that PbCDC42 expression can explain approximately 80% of mother and bud cell shape and 19% of bud cell size. This data support PbCDC42 expression level as being a relevant predictor of P. brasiliensis morphology. Altogether, these fi ndings quantitatively describe the polymorphic nature of the P. brasiliensis yeast form and provide additional support for the key role of PbCDC42 expression on yeast cell morphology. J.F.M. was supported by Fundação para a Ciência e Tecnologia (FCT), Portugal (SFRH/BD/33446/2008).This work was supported by a grant from FCT (PTDC/BIA-MIC/108309/2008). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|