SdiA, an N-Acylhomoserine Lactone Receptor, Becomes Active during the Transit of Salmonella enterica through the Gastrointestinal Tract of Turtles
| Autor: | L. Garry Adams, Brian M. M. Ahmer, James M. Slauch, Craig Altier, Jenee N. Smith, Jessica L. Dyszel, Roy Curtiss, Jitesh A. Soares, Craig D. Ellermeier, Sara D. Lawhon, Vjollca Konjufca |
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| Rok vydání: | 2008 |
| Předmět: |
Salmonella
Guinea Pigs lcsh:Medicine Biology medicine.disease_cause Models Biological Microbiology Lactones Mice 03 medical and health sciences Bacterial Proteins Escherichia medicine Animals lcsh:Science Receptor 030304 developmental biology 0303 health sciences Gastrointestinal tract Multidisciplinary 030306 microbiology lcsh:R Microbiology/Medical Microbiology Salmonella enterica food and beverages biochemical phenomena metabolism and nutrition biology.organism_classification Turtles Gastrointestinal Tract Aeromonas hydrophila Aeromonas Mice Inbred CBA Trans-Activators Cattle Female lcsh:Q Rabbits Microbiology/Cellular Microbiology and Pathogenesis Chickens Ecology/Environmental Microbiology Bacteria Research Article |
| Zdroj: | PLoS ONE, Vol 3, Iss 7, p e2826 (2008) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0002826 |
| Popis: | Background LuxR-type transcription factors are typically used by bacteria to determine the population density of their own species by detecting N-acylhomoserine lactones (AHLs). However, while Escherichia and Salmonella encode a LuxR-type AHL receptor, SdiA, they cannot synthesize AHLs. In vitro, it is known that SdiA can detect AHLs produced by other bacterial species. Methodology/Principal Findings In this report, we tested the hypothesis that SdiA detects the AHL-production of other bacterial species within the animal host. SdiA did not detect AHLs during the transit of Salmonella through the gastrointestinal tract of a guinea pig, a rabbit, a cow, 5 mice, 6 pigs, or 12 chickens. However, SdiA was activated during the transit of Salmonella through turtles. All turtles examined were colonized by the AHL-producing species Aeromonas hydrophila. Conclusions/Significance We conclude that the normal gastrointestinal microbiota of most animal species do not produce AHLs of the correct type, in an appropriate location, or in sufficient quantities to activate SdiA. However, the results obtained with turtles represent the first demonstration of SdiA activity in animals. |
| Databáze: | OpenAIRE |
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