Erythropoietin Attenuates Apoptosis After Ischemia-Reperfusion–Induced Renal Injury in Transiently Hyperglycemic Wister Rats
| Autor: | Elenice Deffune, L. Raquel de Carvalho, Yara Marcondes Machado Castiglia, Marjorie de Assis Golim, A. V. G. de Souza, P. T. G. Vianna Filho, C. de Oliveira, Pedro Thadeu Galvão Vianna, A. M. M. Caetano |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2011 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Necrosis Time Factors Kidney Glomerulus Ischemia Apoptosis Kidney Nephrectomy chemistry.chemical_compound Internal medicine medicine Animals Kidney surgery Rats Wistar Erythropoietin Creatinine Transplantation Renal ischemia business.industry medicine.disease Flow Cytometry Recombinant Proteins Rats Epoetin Alfa Disease Models Animal Endocrinology medicine.anatomical_structure Kidney Tubules chemistry Cytoprotection Hyperglycemia Reperfusion Injury Surgery medicine.symptom business Reperfusion injury medicine.drug |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0041-1345 |
| DOI: | 10.1016/j.transproceed.2011.10.049 |
| Popis: | Made available in DSpace on 2013-09-27T14:54:08Z (GMT). No. of bitstreams: 1 WOS000298620200009.pdf: 131868 bytes, checksum: 1b68b3cadfb65167b31bf5125bba3926 (MD5) Previous issue date: 2011-12-01 Made available in DSpace on 2013-09-30T18:16:04Z (GMT). No. of bitstreams: 1 WOS000298620200009.pdf: 131868 bytes, checksum: 1b68b3cadfb65167b31bf5125bba3926 (MD5) Previous issue date: 2011-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:32:18Z No. of bitstreams: 1 WOS000298620200009.pdf: 131868 bytes, checksum: 1b68b3cadfb65167b31bf5125bba3926 (MD5) Made available in DSpace on 2014-05-20T13:32:18Z (GMT). No. of bitstreams: 1 WOS000298620200009.pdf: 131868 bytes, checksum: 1b68b3cadfb65167b31bf5125bba3926 (MD5) Previous issue date: 2011-12-01 Background. Hyperglycemia is associated with a decreased tolerance to ischemia and an increased severity of renal ischemia reperfusion (I/R) injury. It has been suggested that erythropoietin (EPO) attenuates this effect in normoglycemic animals. This study sought to examine the effects of EPO on treatment renal I/R injury (IRI) in transiently hyperglycemic rats.Material and Methods. Twenty-eight male Wister rats anesthetized with isoflurane received glucose (2.5 g.kg(-1) intraperitoneally) before right nephrectomy. They were randomly assigned to four groups: sham operation (S); IRI (ISO); IRI+EPO, (600 UI kg(-1) low-dose EPO [EL]); and IRI+EPO 5000 UI kg(-1) (high-dose EPO [EH]). IRI was induced by a 25-minute period of left renal ischemia followed by reperfusion for 24 hours. Serum Creatinine and glucose levels were measure at baseline (M1), immediately after the ischemic period (M2), and at 24 hours after reperfusion (M3). After sacrificing the animals, left kidney specimens were submitted for histological analysis including flow cytometry to estimate tubular necrosis and the percentages of apoptotic, dead or intact cells.Results. Scr in the ISO group was significantly higher at M3 than among the other groups. Percentages of early apoptotic cells in ISO group were significantly higher than the other groups. Percentages of late apoptotic cells in S and ISO groups were significantly greater than EL and EH groups. However, no significant intergroup differences were observed regarding the incidence of tubular necrosis.Conclusions. Our results suggested that, although not preventing the occurrence of tubular necrosis, EPO attenuated apoptosis and glomerular functional impairment among transiently hyperglycemic rats undergoing an ischemia/reperfusion insult. São Paulo State Univ UNESP, Dept Anesthesil, São Paulo, Brazil São Paulo State Univ UNESP, Dept Anesthesil, São Paulo, Brazil |
| Databáze: | OpenAIRE |
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