Platelet-activating factor plays a role in the mechanism of major histocompatibility complex in T lymphocytes
| Autor: | A. K. Qayumi, M. Nikbakht-sangari, P. A. Keown, V. Duronio, K. Horley |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
CD3 Complex
Swine CD3 T-Lymphocytes Immunology chemical and pharmacologic phenomena Pyridinium Compounds Major histocompatibility complex Lymphocyte Activation chemistry.chemical_compound Tetrahydroisoquinolines MHC class I Cytotoxic T cell Animals Phytohemagglutinins Platelet Activating Factor biology Platelet-activating factor Histocompatibility Antigens Class II General Medicine Flow Cytometry Isoquinolines Molecular biology In vitro chemistry biology.protein Antibody Cytometry Platelet Aggregation Inhibitors Lung Transplantation |
| Zdroj: | Immunological investigations. 28(4) |
| ISSN: | 0882-0139 |
| Popis: | In recent studies, using a swine model of single lung transplantation, we demonstrated that IRI alone increased MHC II expression in the host's peripheral T lymphocytes. The inhibition of increased MHC II expression with TCV-309, a specific platelet-activating factor (PAF) antagonist suggested that PAF might play a role in the mechanism of increased MHC II expression. The purpose of the current study was two fold: 1) to investigate the mechanism of PAF-induced increased expression of MHC II in T lymphocytes, 2) to determine whether a specific PAF-antagonist, TCV-309, is capable of inhibiting the increased expression in an in vitro system. This study was subdivided, using four in vitro conditions: 1) purified resting T cells, 2) purified proliferating T cells, 3) PBL treated with PAF, and 4) PBL preincubated with TCV-309 and treated with PAF. The level of MHC II on T cells were measured by two color flow cytometry analysis (swine anti-CD3, MHC II-DR-(beta)antibodies). Both MHC II intensity and the number of CD3+MHC+ T cells did not change in resting purified T cells once treated with PAF, Furthermore, MHC II intensity did not change in purified proliferating T cells treated with PAF. The number of CD3+MHC+ T cells, however, increased significantly (p0.05) from day 1 to day 4 as compared with pre-treatment value (day 0) for purified proliferating T cells. Treatment of PBL with PAF (10(-7)M) resulted in a significant (p0.05) increase in MHC II expression from day 2 to day 4 post-treatment. The number of CD3+MHC+ T cells in PBL, however, did not change significantly upon treatment with PAF. The results of this study indicated that PAF did not have a direct effect on increased MHC II expression in resting or proliferating purified T lymphocytes. However, the mechanism of PAF-induced increased expression of MHC II in T cells may be via an indirect pathway involving accessory cells. TCV-309, a specific PAF receptor antagonist, is capable of inhibiting this PAF-induced increased expression of MHC II in T cells. |
| Databáze: | OpenAIRE |
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