Organoid technology for retinal repair
| Autor: | Sílvia Llonch, Madalena Carido, Marius Ader |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Retinal degeneration genetic structures pharmacology [Culture Media Serum-Free] transplantation [Pluripotent Stem Cells] Culture Media Serum-Free Tissue Culture Techniques Translational Research Biomedical chemistry.chemical_compound transplantation [Organoids] Mice cytology [Embryonic Stem Cells] Morphogenesis Cellular Reprogramming Techniques Induced pluripotent stem cell Translational Medical Research Retinal Degeneration cytology [Retina] Organoids medicine.anatomical_structure drug effects [Embryonic Stem Cells] Photoreceptor Cells Vertebrate Pluripotent Stem Cells Induced Pluripotent Stem Cells Context (language use) transplantation [Photoreceptor Cells Vertebrate] Biology Retina 03 medical and health sciences therapy [Retinal Degeneration] Species Specificity ddc:570 Retinitis pigmentosa medicine Animals Humans Molecular Biology Embryonic Stem Cells transplantation [Induced Pluripotent Stem Cells] Retinal Cell Biology Macular degeneration medicine.disease eye diseases Transplantation 030104 developmental biology chemistry sense organs Neuroscience Developmental Biology |
| Zdroj: | Developmental biology 433(2), 132-143 (2018). doi:10.1016/j.ydbio.2017.09.028 |
| ISSN: | 1095-564X |
| DOI: | 10.1016/j.ydbio.2017.09.028 |
| Popis: | A major cause for vision impairment and blindness in industrialized countries is the loss of the light-sensing retinal tissue in the eye. Photoreceptor damage is one of the main characteristics found in retinal degeneration diseases, such as Retinitis Pigmentosa or age-related macular degeneration. The lack of effective therapies to stop photoreceptor loss together with the absence of significant intrinsic regeneration in the human retina converts such degenerative diseases into permanent conditions that are currently irreversible. Cell replacement by means of photoreceptor transplantation has been proposed as a potential approach to tackle cell loss in the retina. Since the first attempt of photoreceptor transplantation in humans, about twenty years ago, several research groups have focused in the development and improvement of technologies necessary to bring cell transplantation for retinal degeneration diseases to reality. Progress in recent years in the generation of human tissue derived from pluripotent stem cells (PSCs) has significantly improved our tools to study human development and disease in the dish. Particularly the availability of 3D culture systems for the generation of PSC-derived organoids, including the human retina, has dramatically increased access to human material for basic and medical research. In this review, we focus on important milestones towards the generation of transplantable photoreceptor precursors from PSC-derived retinal organoids and discuss recent pre-clinical transplantation studies using organoid-derived photoreceptors in context to related in vivo work using primary photoreceptors as donor material. Additionally, we summarize remaining challenges for developing photoreceptor transplantation towards clinical application. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect