Effect of F1 and F2 generations on genetic variability and working steps of doubled haploid production in maize

Autor: Ítalo Stefanine Correia Granato, Roberto Fritsche-Neto, Evellyn Giselly de Oliveira Couto, Deoclécio Domingos Garbuglio, José Crossa, Juan Burgueño, Mayara Neves Cury, Massaine Bandeira e Souza, Miriam Suzane Vidotti
Rok vydání: 2019
Předmět:
Germplasm
Humoral Immune Response
Plant Science
Haploidy
Medicine and Health Sciences
CROMOSSOMOS VEGETAIS
Flowering Plants
education.field_of_study
Multidisciplinary
Plant Anatomy
Eukaryota
Agriculture
Plants
Experimental Organism Systems
Seeds
Medicine
Ploidy
Research Article
Genotype
VARIAÇÃO GENÉTICA EM PLANTAS
Science
Population
Immunology
Biology
Research and Analysis Methods
Zea mays
Chromosomes
Plant
Animal science
Model Organisms
Meiosis
Plant and Algal Models
Genetic variation
Genetics
Genetic variability
Plant breeding
Grasses
education
Alleles
Evolutionary Biology
Population Biology
Organisms
Immunity
Biology and Life Sciences
Genetic Variation
Agronomy
Maize
Plant Breeding
Seedlings
Genetic Loci
Humoral Immunity
Doubled haploidy
Animal Studies
Genetic Polymorphism
Population Genetics
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
PLoS ONE
PLoS ONE, Vol 14, Iss 11, p e0224631 (2019)
Popis: For doubled haploid (DH) production in maize, F1 generation has been the most frequently used for haploid induction due to facility in the process. However, using F2 generation would be a good alternative to increase genetic variability owing to the additional recombination in meiosis. Our goals were to compare the effect of F1 and F2 generations on DH production in tropical germplasm, evaluating the R1-navajo expression in seeds, the working steps of the methodology, and the genetic variability of the DH lines obtained. Sources germplasm in F1 and F2 generations were crossed with the tropicalized haploid inducer LI-ESALQ. After harvest, for both induction crosses were calculated the haploid induction rate (HIR), diploid seed rate (DSR), and inhibition seed rate (ISR) using the total number of seeds obtained. In order to study the effectiveness of the DH working steps in each generation, the percentage per se and the relative percentage were verified. In addition, SNP markers were obtained for genetic variability studies. Results showed that the values for HIR, ISR, and DSR were 1.23%, 23.48%, and 75.21% for F1 and 1.78%, 15.82%, and 82.38% for F2, respectively. The effectiveness of the DH working step showed the same percentage per se value (0.4%) for F1 and F2, while the relative percentage was 27.2% for F1 and 22.4% for F2. Estimates of population parameters in DH lines from F1 were higher than F2. Furthermore, population structure and kinship analyses showed that one additional generation was not sufficient to create new genotype subgroups. Additionally, the relative efficiency of the response to selection in the F1 was 31.88% higher than F2 due to the number of cycles that are used to obtain the DH. Our results showed that in tropical maize, the use of F1 generation is recommended due to a superior balance between time and genetic variability.
Databáze: OpenAIRE
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