Diversity and biosynthetic potential of culturable aerobic heterotrophic bacteria isolated from Magura Cave, Bulgaria
| Autor: | Iva Tomova, Evgenia Vasileva-Tonkova, Irina Lazarkevich, Anna Tomova, Margarita Kambourova |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
QE1-996.5
antimicrobial activity biology culturable bacteria Firmicutes QH301-705.5 Magura Cave Bacillus Bacteroidetes enzyme production Geology Enterobacter biology.organism_classification Actinobacteria Microbiology caves 16S rDNA Botany Stenotrophomonas Proteobacteria hemolytic activity Biology (General) Bacteria Earth-Surface Processes |
| Zdroj: | International Journal of Speleology, Vol 42, Iss 1, Pp 65-76 (2013) |
| ISSN: | 0392-6672 |
| Popis: | Biocapacity of bacteria inhabiting karstic caves to produce valuable biologically active compounds is still slightly investigated. A total of 46 culturable heterotrophic bacteria were isolated under aerobic conditions from the Gallery with pre-historical drawings in Magura Cave, Bulgaria. Phylogenetic analysis revealed that most of bacterial isolates aff iliated with Proteobacteria (63%), followed by Actinobacteria (10.9%), Bacteroidetes (10.9%), and Firmicutes (6.5%). A strong domination of Gram-negative bacteria (total 81%) belonging to nine genera: Serratia, Pseudomonas, Enterobacter, Sphingobacterium, Stenotrophomonas, Commamonas, Acinetobacter, Obesumbacterium, and Myroides, was observed. Gram-positive isolates were represented by the genera Bacillus, Arthrobacter, and Micrococcus. One isolate showed a signif icant phylogenetic distance to the closest neighbor and could represent а novel species. Heterotrophic bacterial isolates from Magura Cave were investigated for hydrolytic enzymes production, antimicrobial and hemolytic activity. Predominance of producers of protease (87%), followed by xanthan lyase (64%), lipase (40%), β-glycosidase (40%), and phytase (21%) was observed. Over 75% of the isolates demonstrated antimicrobial and hemolytic activity. The results suggest that heterotrophic bacteria isolated from Magura Cave could be a valuable source of industrially relevant psychrotolerant enzymes and bioactive metabolites. This study is a f irst report on the taxonomic composition and biological activity of culturable bacteria inhabiting a cave in Bulgaria. |
| Databáze: | OpenAIRE |
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