Sam68 is Overexpressed in Epithelial Ovarian Cancer and Promotes Tumor Cell Proliferation
| Autor: | Hailuo Che, Xuepeng Li, Mingmei Li, Lijuan Dong |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
0301 basic medicine Oncology medicine.medical_specialty endocrine system diseases Cell Cycle Proteins Carcinoma Ovarian Epithelial Biology Pathogenesis 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Risk Factors Lab/In Vitro Research Cell Line Tumor Internal medicine Biomarkers Tumor medicine Carcinoma Humans Neoplasms Glandular and Epithelial RNA Messenger Adaptor Proteins Signal Transducing Cell Proliferation Ovarian Neoplasms Gene knockdown Cell growth RNA-Binding Proteins Cancer General Medicine Middle Aged Prognosis medicine.disease female genital diseases and pregnancy complications DNA-Binding Proteins 030104 developmental biology Real-time polymerase chain reaction Cell culture 030220 oncology & carcinogenesis Female Biological Markers |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| DOI: | 10.12659/msm.899980 |
| Popis: | BACKGROUND Epithelial ovarian cancer (EOC) is the deadliest gynecological malignancy, and evidence is accumulating on how molecular markers may be associated with the origin and process of EOC. Sam68 (Src-associated in mitosis, of 68 kD), is a K homology domain RNA-binding protein that has been investigated as a risk factor in multiple types of tumors. The aim of the present study was to investigate the contribution of the Sam68 gene in the pathogenesis of EOC. MATERIAL AND METHODS Western blot assay and real-time quantitative PCR methods were performed to examine Sam68 expression in EOC tissue specimens. The association of Sam68 expression with clinic-pathologic variables of EOC was evaluated. Then gain-of-function and loss-of-function strategies were adopted to examine the regulation of Sam68 on the proliferation of EOC OVCAR-3 cells using CCK-8 and colony forming assays. RESULTS Sam68 was overexpressed in both mRNA and protein levels in EOC tumor tissue (n=152) in an association with malignant factors of EOC such as International Federation of Gynecology and Obstetrics (FIGO) stage, residual tumor size (cm), histological grade, and lymph node metastasis. In vitro results demonstrated that Sam68 overexpression was upregulated while Sam68 knockdown downregulated the proliferation of EOC OVCAR-3 cells via regulation of cell growth and colony formation. CONCLUSIONS Sam68 was overexpressed in EOC tissue in association with such cancer malignant factors of FIGO stage, histological grade, and lymph node metastasis, and also positively regulated the proliferation of EOC cells. Our research suggests that Sam68 might accelerate cell cycle progression, and present as a prognostic marker for EOC. |
| Databáze: | OpenAIRE |
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