Evaluation of imazethapyr-induced DNA oxidative damage by alkaline Endo III- and Fpg-modified single-cell gel electrophoresis assay in Hypsiboas pulchellus tadpoles (Anura, Hylidae)
| Autor: | Marcelo L. Larramendy, Celeste Ruiz de Arcaute, Sonia Soloneski, Guillermo Sebastián Natale, Juan Manuel Pérez-Iglesias |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis Biología Modified comet assay 010501 environmental sciences medicine.disease_cause 01 natural sciences DNA-formamidopyrimidine glycosylase purl.org/becyt/ford/1 [https] chemistry.chemical_compound Gel electrophoresis Escherichia coli Proteins General Medicine Química Pollution FPG Fpg DNA-Formamidopyrimidine Glycosylase Larva Comet Assay Herbicide Anura Oxidation-Reduction CIENCIAS NATURALES Y EXACTAS Imazethapyr-based formulation MODIFIED COMET ASSAY DNA damage Otras Ciencias Biológicas Endo III Biology OXIDATIVE DNA DAMAGE Ciencias Biológicas 03 medical and health sciences Deoxyribonuclease (Pyrimidine Dimer) medicine Animals purl.org/becyt/ford/1.6 [https] HERBICIDE 0105 earth and related environmental sciences Herbicides Public Health Environmental and Occupational Health Nicotinic Acids IMAZETHAPYR-BASED FORMULATION Molecular biology Comet assay Oxidative Stress 030104 developmental biology chemistry DNA glycosylase Oxidative DNA damage ENDO III Oxidative stress DNA Genotoxicity Water Pollutants Chemical DNA Damage Mutagens |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| Popis: | Imazethapyr (IMZT) is a selective postemergent herbicide with residual action. Available data analyzing its effects in aquatic vertebrates are scarce. In previous studies, we demonstrated that IMZT induces lesions into the DNA of Hypsiboas pulchellus tadpoles using the single-cell gel electrophoresis (SCGE) assay as a biomarker for genotoxicity. Currently, this assay can be modified by including incubation with lesion-specific endonucleases, e.g., endonuclease III (Endo III) and formamidopyrimidine-DNA glycosylase (Fpg), which detect oxidized pyrimidine and purine bases, respectively. The aim of this study was to evaluate the role of oxidative stress in the genotoxic damage in circulating blood cells of H. pulchellus tadpoles exposed to the IMZT-based Pivot H® formulation (10.59% IMZT) at a concentration equivalent to 25% of the LC50 (96 h) value (0.39 mg/L IMZT) during 48 and 96 h. Our results demonstrate that the herbicide induces oxidative DNA damage on H. pulchellus tadpoles at purines bases but not at pyrimidines. Our findings represent the first evidence of oxidative damage caused by IMZT on anuran DNA using the alkaline restriction enzyme-modified SCGE assay. Centro de Investigaciones del Medioambiente Facultad de Ciencias Naturales y Museo |
| Databáze: | OpenAIRE |
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