All trans retinoic acid depresses the content and activity of the mitochondrial ATP synthase in human keratinocytes
| Autor: | Antonio Gnoni, Francesco Papa, Rosa Lippolis, Nicola Sardaro, Salvatore Scacco |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Keratinocytes
Proteomics 0301 basic medicine Protein subunit Biophysics Retinoic acid Down-Regulation Tretinoin Oxidative phosphorylation Inhibitory postsynaptic potential Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Humans Molecular Biology Cells Cultured Cell Proliferation chemistry.chemical_classification Dose-Response Relationship Drug Mass spectrometry ATP synthase biology All-trans retinoic acid Cell growth Human keratinocytes Cell Biology Mitochondrial Proton-Translocating ATPases Molecular biology Enzyme Activation 030104 developmental biology Enzyme chemistry 030220 oncology & carcinogenesis biology.protein |
| Zdroj: | Biochemical and biophysical research communications 482 (2017): 301–304. doi:10.1016/j.bbrc.2016.11.058 info:cnr-pdr/source/autori:Papa, F.; Lippolis, R.; Sardaro, N.; Gnoni, A.; Scacco, S./titolo:All trans retinoic acid depresses the content and activity of the mitochondrial ATP synthase in human keratinocytes/doi:10.1016%2Fj.bbrc.2016.11.058/rivista:Biochemical and biophysical research communications (Print)/anno:2017/pagina_da:301/pagina_a:304/intervallo_pagine:301–304/volume:482 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2016.11.058 |
| Popis: | Proteomic analysis shows that treatment of keratinocytes cultures with all trans retinoic acid (ATRA), under condition in which it inhibits cell growth, results in marked decrease of the level of the F1-beta subunit of the catalytic sector of the mitochondrial FoF1 ATP synthase complex. Enzymatic analysis shows in ATRA-treated keratinocytes a consistent depression of the ATPase activity, with decreased olygomycin sensitivity, indicating an overall alteration of the ATP synthase complex. These findings, together with the previously reported inhibition of respiratory complex I, show that depression of the activity of oxidative phosphorylation enzymes is involved in the cell growth inhibitory action of ATRA. (C) 2016 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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