Gold-Nanorod-Enhanced Fluorescence Correlation Spectroscopy of Fluorophores with High Quantum Yield in Lipid Bilayers
| Autor: | Ankur Gupta, Biswajit Pradhan, Thijs J. Aartsma, Michel Orrit, Gerard W. Canters, Saumyakanti Khatua |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Biomolecule Analytical chemistry Substrate (chemistry) Quantum yield Fluorescence correlation spectroscopy 02 engineering and technology Fluorescence in the life sciences 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials General Energy chemistry Biophysics Molecule lipids (amino acids peptides and proteins) Physical and Theoretical Chemistry 0210 nano-technology Lipid bilayer |
| Zdroj: | The Journal of Physical Chemistry C. 120:25996-26003 |
| ISSN: | 1932-7455 1932-7447 |
| DOI: | 10.1021/acs.jpcc.6b07875 |
| Popis: | Plasmonic fluorescence enhancement is used to study fluorescence correlation spectroscopy (FCS) at higher concentrations than in regular diffraction-limited FCS experiments. Previous studies suffered from sticking to the substrate and were performed mainly with poorly emitting dyes. A lipid bilayer forms a passivating surface, preventing sticking of the dye or the protein, and allows for specific anchoring of probe molecules. For dyes with high quantum yields, the fluorescence background of unenhanced molecules is high, and the fluorescence enhancement is weak, less than about 10. Nonetheless, we show that FCS is possible at micromolar concentrations of the probe molecule. Enhanced FCS is recorded by selecting signals on the basis of their shortened lifetime. This selection enhances the contrast of the correlation by more than an order of magnitude. The lipid bilayer can be used to anchor biomolecules and perform enhanced FCS, as we show for a dye-labeled protein. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|