DNA barcodes of Rosy Tetras and allied species (Characiformes: Characidae: Hyphessobrycon) from the Brazilian Amazon basin

Autor: Jacqueline da Silva Batista, Francis Paola Castro Paz, Jorge Ivan Rebelo Porto
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Evolutionary Genetics
Species Differentiation
Hyphessobrycon
lcsh:Medicine
Fresh Water
Characiformes
Procedures
Geographical Variation (species)
DNA barcoding
Hyphessobrycon Rosaceus
Monophyly
Molecular Systematics
Haplotype
lcsh:Science
Phylogeny
River
Animalss
Multidisciplinary
biology
Coi Gene
Cytochrome C Oxidase
Hyphessobrycon Pulchrippinis
Fishes
Freshwater Fish
Hyphessobrycon Copelandi
Genetic Variability
Taxonomy (biology)
Sequence Analysis
Brazil
Research Article
Hyphessobrycon Bentosi
Fish Biology
Dna Barcoding
Taxonomic
Zoology
Hyphessobrycon Epicharis
Cladistics
Hyphessobrycon Eques
Rivers
River Basin
Genetics
DNA Barcoding
Taxonomic
Animals
Evolutionary Systematics
Controlled Study
Molecular Biology Techniques
Sequencing Techniques
Molecular Biology
Taxonomy
Evolutionary Biology
Hyphessobrycon Erythrostigma
Rosy tetra
Characidae
Dna Barcoding
Brasil
lcsh:R
Biology and Life Sciences
Computational Biology
DNA
Dna
Nonhuman
biology.organism_classification
Hyphessobrycon Sweglesi
Fish
Animal Taxonomy
Neighbor Joining Method
Species Identification
Hyphessobrycon Pyrrhonotus
lcsh:Q
Species richness
Animal Genetics
Hyphessobrycon Socolofi
Zdroj: PLoS ONE, Vol 9, Iss 5, p e98603 (2014)
Repositório Institucional do INPA
Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
PLoS ONE
ISSN: 1932-6203
Popis: DNA barcoding can be an effective tool for fast and accurate species-level identification based on sequencing of the mitochondrial cytochrome c oxidase subunit (COI) gene. The diversity of this fragment can be used to estimate the richness of the respective species. In this study, we explored the use of DNA barcoding in a group of ornamental freshwater fish of the genus Hyphessobrycon. We sequenced the COI from 10 species of Hyphessobrycon belonging to the "Rosy Tetra Clade" collected from the Amazon and Negro River basins and combined our results with published data. The average conspecific and congeneric Kimura 2-parameter distances were 2.3% and 19.3%, respectively. Six of the 10 species were easily distinguishable by DNA barcoding (H. bentosi, H. copelandi, H. eques, H. epicharis, H. pulchrippinis, and H. sweglesi), whereas the remaining species (H. erythrostigma, H. pyrrhonotus, H. rosaceus and H. socolofi) lacked reciprocal monophyly. Although the COI gene was not fully diagnostic, the discovery of distinct evolutionary units in certain Hyphessobrycon species under the same specific epithet as well as haplotype sharing between different species suggest that DNA barcoding is useful for species identification in this speciose genus. © 2014 Castro Paz et al.
Databáze: OpenAIRE
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