Insulin-induced lipid binding to hemoglobin
| Autor: | Milan Nikolić, Karen Klappe, Dick Hoekstra, Nenad Tomašević, Vesna Niketić |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
insulin
media_common.quotation_subject Membrane lipids medicine.medical_treatment ERYTHROCYTE-MEMBRANE LIPOSOMES hyperinsulinism OXIDATION PEROXIDATION PATHWAY lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine PHOSPHOLIPID-VESICLES medicine hemoglobin Internalization 030304 developmental biology media_common 0303 health sciences Liposome RECEPTOR Chemistry Cholesterol Insulin membrane lipids General Chemistry Phosphatidylserine medicine.disease hypoglycemia lcsh:QD1-999 Biochemistry 030220 oncology & carcinogenesis INTERNALIZATION lipids (amino acids peptides and proteins) erythrocyte Hemoglobin Hyperinsulinism |
| Zdroj: | Journal of the Serbian Chemical Society, Vol 68, Iss 1, Pp 25-34 (2003) Journal of the serbian chemical society, 68(1), 25-33 Journal of the Serbian Chemical Society, Vol 68, Iss 1, Pp 25-33 (2003) |
| ISSN: | 1820-7421 0352-5139 |
| Popis: | Under hypoglycemic conditions, concomitant hyperinsulinism causes an apparent modification of hemoglobin (Hb) which is manifested by its aggregation (Niketic et al., Clin. Chim. Acta 197 (1991) 47). In the present work the causes and mechanisms underlying this Hb modification were studied. Hemoglobin isolated from normal erythrocytes incubated with insulin was analyzed by applying 31P-spectrometry and lipid extraction and analysis. To study the dynamics of the plasma membrane during hyperinsulinism, a fluorescent lipid-analog was applied. In the presence of insulin phosphatidylserine (PS), phosphatidylethanolamine (PE) and cholesterol were found to bind to Hb. Lipid binding resulted in Hb aggregation, a condition that can be reproduced when phospholipids are incubated with Hb in vitro. Using a fluorescent lipid-analog, it was also shown that exposing erythrocytes to supraphysiological concentrations of insulin in vitro resulted in the internalization of lipids. The results presented in this work may have relevance to cases of diabetes mellitus and hypoglycemia. |
| Databáze: | OpenAIRE |
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