Matrine inhibits proliferation and induces apoptosis via BID-mediated mitochondrial pathway in esophageal cancer cells
| Autor: | Haoxin Du, Huan Zhou, Hanwei Cao, Tianhui Hu, Qiao Wang, Bing Zhang, Guojun Geng, Minying Xu, Gang Song |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Cell cycle checkpoint Esophageal Neoplasms Cell Survival Cell Antineoplastic Agents Apoptosis Mitochondrion Models Biological chemistry.chemical_compound Mice Alkaloids Matrine In vivo Cell Line Tumor Genetics medicine Animals Humans Viability assay Matrines Molecular Biology Cell Proliferation Caspase 8 Chemistry Cytochromes c General Medicine Cell Cycle Checkpoints Xenograft Model Antitumor Assays Caspase 9 Mitochondria Tumor Burden Disease Models Animal Protein Transport medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Cell culture Immunology Cancer research Poly(ADP-ribose) Polymerases Quinolizines BH3 Interacting Domain Death Agonist Protein Signal Transduction |
| Zdroj: | Molecular biology reports. 41(5) |
| ISSN: | 1573-4978 |
| Popis: | Matrine, as a member of Sophora family, is an alkaloid found in plants, and produces plethora pharmacological effects, including anti-cancer effects. However, the mechanism involved remains largely unknown. This study is conducted to investigate the anti-cancer mechanisms of matrine in human esophageal cancer in vitro and in vivo. In human esophageal cancer cell Eca-109, matrine significantly decreased the cell viability in a dose-dependent manner, and induced apoptosis as well as cell cycle arrest in G0/G1 phase by up-regulation of P53 and P21. The expression of several apoptosis-related proteins in cells and tumor tissues were evaluated by Western blot analysis. We found that matrine induced cell apoptosis by down-regulation of the ratio of BCL-2/BID and increasing activation of caspase-9. Further studies indicated that matrine induced apoptosis of Eca-109 was through the mitochondria-mediated internal pathway, but not by death receptor-mediated extrinsic apoptotic pathway, which was confirmed by the fact that Bid translocated from the nucleus to mitochondria during the process of the apoptosis induced by matrine. In vivo study found that matrine effectively inhibited the tumor formation of Eca-109 cells in nude mice. Our study suggests that matrine could serve as a potential novel agent from natural products to treat esophageal cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink