Identification of a Chlamydomonas plastidial 2-lysophosphatidic acid acyltransferase and its use to engineer microalgae with increased oil content
| Autor: | Yeongho Kim, Miriam Schulz-Raffelt, Shogo Kamisuki, Yonghua Li-Beisson, Sunghoon Jang, Youngsook Lee, Won-Yong Song, Donghwi Ko, Bertrand Legeret, Yasuyo Yamaoka, Dorine Achard, Ikuo Nishida |
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| Přispěvatelé: | Pohang University of Science and Technology (POSTECH), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Environnement, Bioénergie, Microalgues et Plantes (EBMP), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Saitama University, Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), ANR-13-JSV5-0005,MUsCA,Ingénierie métabolique d'une microalgue verte en vue de la production d'alcanes à chaine moyenne(2013), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Bioénergie et Microalgues (EBM) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine lysophosphatidic acid acyltransferase [SDV]Life Sciences [q-bio] Chlamydomonas reinhardtii Plastid membrane Plant Science 01 natural sciences 03 medical and health sciences chemistry.chemical_compound triacylglycerols Plant Oils Plastids Plastid Research Articles ComputingMilieux_MISCELLANEOUS biology microalgae Chlamydomonas Phosphatidic acid biology.organism_classification Enzyme assay 030104 developmental biology Biochemistry chemistry acyl specificity Acyltransferases plastid transformation oil content Acyltransferase biology.protein Agronomy and Crop Science 010606 plant biology & botany Biotechnology Research Article |
| Zdroj: | Plant Biotechnology Journal Plant Biotechnology Journal, 2016, 14 (11), pp.2158-2167. ⟨10.1111/pbi.12572⟩ PLANT BIOTECHNOLOGY JOURNAL(14): 11 Plant Biotechnology Journal, Wiley, 2016, 14 (11), pp.2158-2167. ⟨10.1111/pbi.12572⟩ |
| ISSN: | 1467-7644 1467-7652 |
| Popis: | Summary Despite a strong interest in microalgal oil production, our understanding of the biosynthetic pathways that produce algal lipids and the genes involved in the biosynthetic processes remains incomplete. Here, we report that Chlamydomonas reinhardtii Cre09.g398289 encodes a plastid‐targeted 2‐lysophosphatidic acid acyltransferase (CrLPAAT1) that acylates the sn‐2 position of a 2‐lysophosphatidic acid to form phosphatidic acid, the first common precursor of membrane and storage lipids. In vitro enzyme assays showed that CrLPAAT1 prefers 16:0‐CoA to 18:1‐CoA as an acyl donor. Fluorescent protein‐tagged CrLPAAT1 was localized to the plastid membrane in C. reinhardtii cells. Furthermore, expression of CrLPAAT1 in plastids led to a > 20% increase in oil content under nitrogen‐deficient conditions. Taken together, these results demonstrate that CrLPAAT1 is an authentic plastid‐targeted LPAAT in C. reinhardtii, and that it may be used as a molecular tool to genetically increase oil content in microalgae. |
| Databáze: | OpenAIRE |
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