Human umbilical cord hyaluronate neutral sugar content and carbohydrate-protein linkage studies
| Autor: | Ranbir S. Varma, Rajendra Varma, William S. Allen, Ahmad H. Wardi |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Arabinose
Chromatography Gas Optical Rotation Chromatography Paper Biophysics Galactosamine Glucuronates Uronic acid Biochemistry Fucose Umbilical Cord chemistry.chemical_compound Pregnancy Aspartic acid Humans Amino Acids Hyaluronic Acid Threonine Molecular Biology chemistry.chemical_classification Glucosamine Chromatography Viscosity Proteins Hydrogen-Ion Concentration Carbohydrate Amino acid Molecular Weight Paper chromatography Glucose chemistry Female |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 399:139-144 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/0304-4165(75)90220-2 |
| Popis: | Paper chromatography of neutral sugars and gas chromatography of their aldononitrile acetates indicated the presence of fucose, arabinose and a small amount of glucose in purified human umbilical cord hyaluronate. The molar ratios of serine, threonine and aspartic acid to neutral sugars were not unity, suggesting the non-involvement of the neutral sugars and the amino acids in a carbohydrate-protein linkage. The same was indicated by an increase in the percentage of the aforementioned amino acids and by the absence of sugar alditols in umbilical cord hyaluronate reduced eith NaBH4 -PdCl2, after alkali treatment. This reduction caused a decrease in the intrinsic viscosity and molecular wieght to about one-half and an appreciable decrease in the specific rota tion of hyaluronate, suggesting a separation of the two antiparallel chains o the double helical hyaluronate. The umbilical cord hyluronate containe contained bound silicon and it is possible that this bound silicon may cross-link the two chains at interspersed intervals through the uronic acid moiety and/or through neutral sugars. |
| Databáze: | OpenAIRE |
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