cDNA cloning and nucleotide sequence of the wheat streak mosaic virus capsid protein gene
| Autor: | L. A. Calvert, Roger N. Beachy, S. A. Lommel, C. L. Niblett, T. L. Kendall, C. E. Smith, K. R. Zagula, D. M. Stark |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Untranslated region
Transcription Genetic viruses Molecular Sequence Data Biology Capsid Mosaic Viruses Virology Complementary DNA Amino Acid Sequence Cloning Molecular Peptide sequence Wheat streak mosaic virus Genetics Base Sequence Nucleic acid sequence Potyvirus Virion RNA biology.organism_classification Precipitin Tests Protein Biosynthesis DNA Viral RNA Viral Electrophoresis Polyacrylamide Gel Sequence Alignment |
| Zdroj: | The Journal of general virology. 72 |
| ISSN: | 0022-1317 |
| Popis: | The 3′-terminal region of wheat streak mosaic virus (WSMV) genomic RNA was cloned and a cDNA sequence of 1809 nucleotides upstream of the poly(A) tract was determined. The sequence contains a single open reading frame of 1662 nucleotides and a 3′ untranslated region of 147 nucleotides. Translation products from WSMV RNA and WSMV cDNA transcripts were immunoprecipitated by WSMV capsid protein antiserum, indicating that the 3′-terminal region of WSMV RNA encodes the capsid protein. Five potential N-terminal capsid protein protease cleavage sites were identified, which would yield proteins ranging from 31.7K to 46.8K. Alignment of the deduced amino acid sequence of the WSMV capsid protein with those of other potyviruses showed significant, but limited, identity as compared to the alignment of two or more aphid-transmitted potyviruses. Although WSMV has characteristics distinct from poty-viruses, because of its particle morphology, translation strategy apparently based on polyprotein processing, the ability to form cytoplasmic cylindrical inclusions and the degree of capsid protein homology with aphidtransmitted potyviruses, it should be considered a member of the potyvirus group. |
| Databáze: | OpenAIRE |
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