Chemical, physico-chemical and cytotoxicity characterisation of xyloglucan from Guibourtia hymenifolia (Moric.) J. Leonard seeds
| Autor: | Maria Rita Sierakowski, Neoli Lucyszyn, Clayton F. de Souza, Adriana F. Lubambo, Tatiane A. Jó, Lucy Ono |
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| Rok vydání: | 2011 |
| Předmět: |
Molar mass
Hydrodynamic radius biology Hydrocolloids General Chemical Engineering Intrinsic viscosity General Chemistry engineering.material biology.organism_classification Random coil Xyloglucan chemistry.chemical_compound chemistry Polymer chemistry engineering Radius of gyration Biopolymer Polysaccharide Guibourtia hymenifolia (Moric.) J. Leonard Guibourtia Nuclear chemistry Food Science |
| Zdroj: | Food Hydrocolloids. 25(5):1242-1250 |
| ISSN: | 0268-005X |
| DOI: | 10.1016/j.foodhyd.2010.11.012 |
| Popis: | A water-soluble polysaccharide was obtained from Guibourtia hymenifolia seeds in a 54.2% yield (w/w). The Glc:Xyl:Gal molar ratio was 3.3:2.3:1. The methylation results and 1D/2D NMR spectra indicated the presence of xyloglucan (XG), the intrinsic viscosity of which was 665 mL/g. The molar mass ( M w ), radius of gyration (R g ), hydrodynamic radius (R h ), and ρ (R g /R h ) of XG were 8.43 × 10 5 g/mol, 97 nm, 61 nm, and 1.59, respectively, indicating a random coil and flexible conformation that was subsequently confirmed by the Mark–Houwink constant α (0.70). Atomic force microscopy analysis of XG adsorbed on silicon revealed that the chains are an average of 1.25-nm high, 28.9-nm wide and 131-nm long. Furthermore, a cytotoxicity assay indicated a high CC 50 value (>3.3 mg/mL). These results suggest that this biopolymer has potential applications in different food technologies and biotechnological processes. |
| Databáze: | OpenAIRE |
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