Deleterious effects of carbon-carbon dipolar coupling on RNA NMR dynamics
| Autor: | Daniel Oh, Regan M. LeBlanc, Owen Becette, Hyeyeon Nam, David A. Case, Theodore K. Dayie |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Chemistry Biomolecule Relaxation (NMR) chemistry.chemical_element Nuclear magnetic resonance spectroscopy Nuclear Overhauser effect 010402 general chemistry 01 natural sciences Biochemistry Carbon 0104 chemical sciences 03 medical and health sciences 030104 developmental biology Molecular recognition Heteronuclear molecule Chemical physics RNA Carbon-13 Magnetic Resonance Spectroscopy Spectroscopy Magnetic dipole–dipole interaction Density Functional Theory |
| Zdroj: | Journal of biomolecular NMR. 74(6-7) |
| ISSN: | 1573-5001 |
| Popis: | Many regulatory RNAs undergo dynamic exchanges that are crucial for their biological functions and NMR spectroscopy is a versatile tool for monitoring dynamic motions of biomolecules. Meaningful information on biomolecular dynamics requires an accurate measurement of relaxation parameters such as longitudinal (R1) rates, transverse (R2) rates and heteronuclear Overhauser effect (hNOE). However, earlier studies have shown that the large 13C-13C interactions complicate analysis of the carbon relaxation parameters. To investigate the effect of 13C-13C interactions on RNA dynamic studies, we performed relaxation measurements on various RNA samples with different labeling patterns and compared these measurements with the computational simulations. For uniformly labeled samples, contributions of the neighboring carbon to R1 measurements were observed. These contributions increased with increasing magnetic field and overall correlation time ([Formula: see text]) for R1 rates, necessitating more careful analysis for uniformly labeled large RNAs. In addition, the hNOE measurements were also affected by the adjacent carbon nuclei. Unlike R1 rates, R1ρ rates showed relatively good agreement between uniformly- and site-selectively labeled samples, suggesting no dramatic effect from their attached carbon, in agreement with previous observations. Overall, having more accurate rate measurements avoids complex analysis and will be a key for interpreting 13C relaxation rates for molecular motion that can provide valuable insights into cellular molecular recognition events. |
| Databáze: | OpenAIRE |
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