A practical workflow for capillary microsampling in nonclinical studies

Autor:	Raja Mangipudy, Ting Su, Linna Wang, Qin C Ji, Kristina D. Chadwick, Bonnie Wang, Renuka Pillutla
Rok vydání:	2019
Předmět:	Sample handling Medical Laboratory Technology Bioanalysis Workflow Chromatography Materials science Plasma samples Small volume Capillary action Clinical Biochemistry General Medicine General Pharmacology Toxicology and Pharmaceutics Analytical Chemistry
Zdroj:	Bioanalysis. 11:175-184
ISSN:	1757-6199 1757-6180
DOI:	10.4155/bio-2018-0224
Popis:	Aim: The result of investigation on the procedure of sample handling and bioanalysis of small volume of plasma sample for nonclinical studies stored in 0.5-ml micronic tubes was reported. Results/methodology: Sample integrity of the small volume (25 μl) during long-term storage and the feasibility and data reliability of performing multiple re-assays on the small volume sample using 5 μl aliquot per analysis was evaluated. Conclusion: Integrity was maintained in samples (25 μl) stored for up to 1 month in 0.5-ml micronic tubes at -20°C. A 25 μl sample is sufficient for four-times of re-assays. This evaluation demonstrated the feasibility of this workflow of handling and bioanalysis on small volume plasma sample for GLP studies under the US FDA guidance.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e79fcad10850d3f247bf2327e7dd664 https://doi.org/10.4155/bio-2018-0224 Zobrazit plný text záznamu