3-Methylthiopropionic Acid of Rhizoctonia solani AG-3 and Its Role in the Pathogenicity of the Fungus
| Autor: | Haitao Long, Lumei Pu, Huizhen Qiu, Chun-hong Zhang, Jing He, Hui-Xiu Zhang, Frederick Kankam |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:Plant culture stem canker 01 natural sciences Rhizoctonia solani Cell wall 03 medical and health sciences Botany medicine lcsh:SB1-1110 Mycelium Canker phytotoxin biology Stolon food and beverages Phytotoxin biology.organism_classification medicine.disease Horticulture 3-methylthiopropionic acid 030104 developmental biology Cytoplasm Seedling cell membrane Agronomy and Crop Science Research Article 010606 plant biology & botany |
| Zdroj: | The Plant Pathology Journal, Vol 32, Iss 2, Pp 85-94 (2016) The Plant Pathology Journal |
| ISSN: | 1598-2254 |
| DOI: | 10.5423/ppj.oa.08.2015.0159 |
| Popis: | Studies were conducted to determine the role of 3-methylthioproprionic acid (MTPA) in the pathogenicity of potato stem canker, Rhizoctonia solani, and the concentrations required to inhibit growth of R. solani under laboratory and plant house-based conditions. The experiments were laid out in a completely randomized design with five treatments and five replications. The treatments were 0, 1, 2, 4, and 8 mM concentrations of MTPA. The purified toxin exhibited maximal activity at pH 2.5 and 30°C. MTPA at 1, 2, 4, and 8 mM levels reduced plant height, chlorophyll content, haulm fresh weight, number of stolons, canopy development, and tuber weight of potato plants, as compared to the control. MTPA significantly affected mycelial growth with 8 mM causing the highest infection. The potato seedlings treated with MTPA concentrations of 1.0-8.0 mM induced necrosis of up to 80% of root system area. Cankers were resulted from the injection of potato seedling stems with 8.0 mM MTPA. The results showed the disappearance of cell membrane, rough mitochondrial and cell walls, change of the shape of chloroplasts, and swollen endoplasmic reticulum. Seventy-six (76) hours after toxin treatment, cell contents were completely broken, cytoplasm dissolved, and more chromatin were seen in the nucleus. The results suggested that high levels of the toxin concentration caused cell membrane and cytoplasm fracture. The integrity of cellular structure was destroyed by the phytotoxin. The concentrations of the phytotoxin were significantly correlated with pathogenicity and caused damage to the cell membrane of potato stem base tissue. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|