A Novel Glial Growth Inhibitory Factor, Gliostatin, Derived from Neurofibroma
| Autor: | Akihiko Moriyama, Takayoshi Hirano, Shuji Kaneko, Kiyofumi Asai, Ichiro Isobe, Keiko Nakanishi, Taiji Kato, Yaman Z. Eksioglu |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.drug_class Blotting Western Nerve Tissue Proteins Endogeny Glial tumor Biology Monoclonal antibody Biochemistry Mice Cellular and Molecular Neuroscience Column chromatography Western blot Tumor Cells Cultured medicine Animals Mice Inbred BALB C Thymidine Phosphorylase Neurofibroma medicine.diagnostic_test DNA synthesis Tissue Extracts Cell growth Antibodies Monoclonal Molecular biology Blot Female |
| Zdroj: | Journal of Neurochemistry. 59:307-317 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1992.tb08905.x |
| Popis: | Neurofibroma tissue was investigated for the presence of glial growth modulators that would suppress the proliferation of glial cells. A novel endogenous polypeptide inhibitor of proliferation and DNA synthesis in glial cells, gliostatin, was purified from the extracts of neurofibroma by a procedure comprising dye and anion-exchange column chromatography, and HPLC. A monoclonal antibody raised against partially purified gliostatin showed no cross-reactivity with known cytokines, but adsorbed the growth inhibitory activity of gliostatin and immunochemically visualized the putative gliostatin bands on western blot analyses. Although the product showed an apparent M(r) of 100,000 accompanied by an inhibitory activity on gel filtration column chromatography, it migrated at a lower apparent M(r) of 50,000 under the reducing conditions on western blotting, indicating that a homodimeric structure of native gliostatin consisted of 50-kDa subcomponents. Gliostatin was a potent growth inhibitor acting at nanomolar concentrations against all glial tumor cells and glia maturation factor-stimulated astroblasts, but not neuronal cells. |
| Databáze: | OpenAIRE |
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