Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM – a microarray approach
| Autor: | Klaus P. Brüssow, Maciej Zabel, Dorota Bukowska, Małgorzata Bruska, Wiesława Kranc, Bartosz Kempisty, Artur Bryja, Michal Jeseta, Katarzyna Wojtanowicz-Markiewicz, Sylwia Borys, Marta Rybska, Sylwia Ciesiółka, Joanna Budna, Michał Nowicki, Paweł Antosik, Ewa Sumelka |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty lcsh:QH471-489 Swine Microarray Bone morphogenetic protein Oogenesis lcsh:Gynecology and obstetrics 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine Gene expression medicine Animals lcsh:Reproduction BMP signaling pathway lcsh:RG1-991 Pig Cumulus Cells 030219 obstetrics & reproductive medicine biology Gene Expression Profiling Research Gene Expression Regulation Developmental Obstetrics and Gynecology Transforming growth factor beta Microarray Analysis In Vitro Oocyte Maturation Techniques In vitro maturation Cell biology 030104 developmental biology Reproductive Medicine Bone Morphogenetic Proteins embryonic structures biology.protein Oocytes Female Folliculogenesis Transcriptome Signal Transduction Developmental Biology Follistatin |
| Zdroj: | Reproductive Biology and Endocrinology, Vol 15, Iss 1, Pp 1-9 (2017) Reproductive Biology and Endocrinology : RB&E |
| ISSN: | 1477-7827 |
| Popis: | Background The full maturational capability of mammalian oocytes is accompanied by nuclear and cytoplasmic modifications, which are associated with proliferation and differentiation of surrounding cumulus cells. These events are regulated on molecular level by the expression of target genes involved in signal transduction pathways crucial for folliculogenesis and oogenesis. Transforming growth factor beta signaling includes several molecules that are involved in the regulation of oogenesis and embryo growth, including bone morphogenetic protein (BMP). However, the BMP-related gene expression profile in oocytes at different maturational stages requires further investigation. Methods Oocytes were isolated from pubertal crossbred Landrace gilts follicles, selected with a use of BCB staining test and analyzed before and after in vitro maturation. Gene expression profiles were examined using an Affymetrix microarray approach and validated by RT-qPCR. Database for Annotation, Visualization, and Integrated Discovery (DAVID) software was used for the extraction of the genes belonging to a BMP-signaling pathway ontology group. Results The assay revealed 12,258 different transcripts in porcine oocytes, among which 379 genes were down-regulated and 40 were up-regulated. The DAVID database indicated a “BMP signaling pathway” ontology group, which was significantly regulated in both groups of oocytes. We discovered five up-regulated genes in oocytes before versus after in vitro maturation (IVM): chordin-like 1 (CHRDL1), follistatin (FST), transforming growth factor-beta receptor-type III (TGFβR3), decapentaplegic homolog 4 (SMAD4), and inhibitor of DNA binding 1 (ID1). Conclusions Increased expression of CHRDL1, FST, TGFβR3, SMAD4, and ID1 transcripts before IVM suggested a subordinate role of the BMP signaling pathway in porcine oocyte maturational competence. Conversely, it is postulated that these genes are involved in early stages of folliculogenesis and oogenesis regulation in pigs, since in oocytes before IVM increased expression was observed. |
| Databáze: | OpenAIRE |
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