Rare, high-affinity mouse anti-PD-1 antibodies that function in checkpoint blockade, discovered using microfluidics and molecular genomics
| Autor: | Rena A. Mizrahi, Adam S. Adler, Matthew J. Spindler, Matthew Adams, Michael A. Asensio, Robert C. Edgar, David S. Johnson, Jackson Leong, Renee Leong |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.drug_class Microfluidics Programmed Cell Death 1 Receptor Immunology Antibody Affinity chemical and pharmacologic phenomena Genomics Yeast display Monoclonal antibody Deep sequencing deep sequencing Mice 03 medical and health sciences 0302 clinical medicine Peptide Library Report PD-1 medicine Animals Humans Immunology and Allergy yeast display Hybridomas biology Antibodies Monoclonal High-Throughput Nucleotide Sequencing Cell sorting Flow Cytometry Complementarity Determining Regions Molecular biology Yeast 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Antibody checkpoint inhibitors mouse repertoire Function (biology) Protein Binding Single-Chain Antibodies |
| Zdroj: | mAbs |
| ISSN: | 1942-0870 1942-0862 |
| DOI: | 10.1080/19420862.2017.1371386 |
| Popis: | Conventionally, mouse hybridomas or well-plate screening are used to identify therapeutic monoclonal antibody candidates. In this study, we present an alternative to hybridoma-based discovery that combines microfluidics, yeast single-chain variable fragment (scFv) display, and deep sequencing to rapidly interrogate and screen mouse antibody repertoires. We used our approach on six wild-type mice to identify 269 molecules that bind to programmed cell death protein 1 (PD-1), which were present at an average of 1 in 2,000 in the pre-sort scFv libraries. Two rounds of fluorescence-activated cell sorting (FACS) produced populations of PD-1-binding scFv with a mean enrichment of 800-fold, whereas most scFv present in the pre-sort mouse repertoires were de-enriched. Therefore, our work suggests that most of the antibodies present in the repertoires of immunized mice are not strong binders to PD-1. We observed clusters of related antibody sequences in each mouse following FACS, suggesting evolution of clonal lineages. In the pre-sort repertoires, these putative clonal lineages varied in both the complementary-determining region (CDR)3K and CDR3H, while the FACS-selected PD-1-binding subsets varied primarily in the CDR3H. PD-1 binders were generally not highly diverged from germline, showing 98% identity on average with germline V-genes. Some CDR3 sequences were discovered in more than one animal, even across different mouse strains, suggesting convergent evolution. We synthesized 17 of the anti-PD-1 binders as full-length monoclonal antibodies. All 17 full-length antibodies bound recombinant PD-1 with KD < 500 nM (average = 62 nM). Fifteen of the 17 full-length antibodies specifically bound surface-expressed PD-1 in a FACS assay, and nine of the antibodies functioned as checkpoint inhibitors in a cellular assay. We conclude that our method is a viable alternative to hybridomas, with key advantages in comprehensiveness and turnaround time. |
| Databáze: | OpenAIRE |
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