In Vitro Interactions of Biomedical Polyurethanes with Macrophages and Bacterial Cells
| Autor: | M. Cristina Tanzi, Paola Petrini, Silvia Farè, Livia Visai, Pietro Speziale, Simonetta Rindi |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Staphylococcus aureus
Materials science Biocompatibility Polyurethanes 0206 medical engineering Biomedical Engineering Biotin Enzyme-Linked Immunosorbent Assay 02 engineering and technology In Vitro Techniques medicine.disease_cause Cell Line Microbiology Biomaterials Staphylococcus epidermidis Cell Adhesion medicine Cell adhesion biology Macrophages macrophage adhesion Fibrinogen Adhesion bacterial adhesion bacterial infections and mycoses 021001 nanoscience & nanotechnology biology.organism_classification 020601 biomedical engineering Blood proteins In vitro Fibronectins Fibronectin polyurethane protein coating Microscopy Electron Scanning biology.protein 0210 nano-technology |
| Zdroj: | Politecnico di Milano-IRIS |
| ISSN: | 1530-8022 0885-3282 |
| DOI: | 10.1177/0885328202016003175 |
| Popis: | Three commercial medical-grade polyurethanes (PUs), a poly-ether-urethane (Pellethane), and two poly-carbonate-urethanes, the one aromatic (Bionate) and the other aliphatic (Chronoflex), were tested for macrophages and bacterial cells adhesion, in the presence or absence of adhesive plasma proteins. All the experiments were carried out on PUs films obtained by solvent casting. The wettability of these films was analysed by measuring static contact angles against water. The ability of the selected PUs to adsorb human fibronectin (Fn) and fibrinogen (Fbg) was checked by ELISA with biotin-labelled proteins. All PUs were able to adsorb Fn and Fbg(Fn > Fbg). Fn adsorption was in the order: Pellethane > Chronoflex > Bionate, the highest Fbg adsorption being detected onto Bionate (Bionate > Chronoflex > Pellethane). The human macrophagic line J111, and the two main bacterial strains responsible for infection in humans (Staphylococcus aureus Newman and Staphylococcus epidermidis 14852) were incubated in turn with the three PUs, uncoated or coated with plasma proteins. No macrophage or bacterial adhesion was observed onto uncoated PUs. PUs coated with plasma, Fn or Fbgpromoted bacterial adhesion (S. aureus > S. epidermidis), whereas macrophage adhered more onto PUs coated with Fn or plasma. The coating with Fbg did not promote cell adhesion. Pellethane showed the highest macrophage activation (i.e. spreading), followed, in the order, by Bionate and Chronoflex. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|