Effects of 24R,25-Dihydroxyvitamin D3 on Alkaline Phosphatase Activity in Pig Renal Epithelial LLC-PK1 Cells in Culture
| Autor: | B. Do, J. R. Claude, D. Durand, R. Breget, Jean-Michel Warnet, X. Do Thanh, F. Massicot, N. Taniguchi, J. P. Clot |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Phosphoric monoester hydrolases 24 25-Dihydroxyvitamin D 3 Swine Metabolite Acid Phosphatase Biology Kidney Tubules Proximal chemistry.chemical_compound Enzyme activator stomatognathic system Internal medicine medicine Animals Enzyme inducer Pharmacology Kidney Microvilli Cell Membrane Acid phosphatase Epithelial Cells gamma-Glutamyltransferase Alkaline Phosphatase Hormones Rats Enzyme Activation Endocrinology medicine.anatomical_structure chemistry Cell culture Enzyme Induction biology.protein LLC-PK1 Cells Alkaline phosphatase |
| Zdroj: | General Pharmacology: The Vascular System. 31:459-462 |
| ISSN: | 0306-3623 |
| DOI: | 10.1016/s0306-3623(98)00011-1 |
| Popis: | 1. The effects of 24R,25-dihydroxyvitamin D3 [24,25(OH)2D3] on alkaline phosphatase activity (ALP) were evaluated in pig kidney LLC-PK1 cells in culture. 2. The vitamin D3 metabolite increased ALP activity in these cells, whereas no effect of the hormone was observed on gamma-glutamyltranspeptidase and acid phosphatase activities. 3. ALP activity was stimulated after 3- to 12-hr incubation in the presence of 10(-9) mol/l 24,25(OH)2D3 with a maximum after 6 hr. 4. The hormonal induction of ALP activity was prevented by pretreatment of cells by actinomycin D. 5. It is proposed that 24,25(OH)2D3 could increase ALP activity by de novo protein synthesis. |
| Databáze: | OpenAIRE |
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